Effect of Bariatric Surgery-Induced Weight Loss on SR-BI-, ABCG1-, and ABCA1-Mediated Cellular Cholesterol Efflux in Obese Women

• Published in: The journal of clinical endocrinology and metabolism Vol. 96; no. 4; pp. 1151 - 1159
• Main Authors: Aron-Wisnewsky, Judith, Julia, Zélie, Poitou, Christine, Bouillot, Jean-Luc, Basdevant, Arnaud, Chapman, M. John, Clement, Karine, Guerin, Maryse
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Oxford University Press 01.04.2011; Copyright by The Endocrine Society; Endocrine Society
• Subjects: ABCA1 protein; Adolescent; Adult; Animals; ATP Binding Cassette Transporter 1; ATP Binding Cassette Transporter, Sub-Family G, Member 1; ATP-Binding Cassette Transporters - genetics; ATP-Binding Cassette Transporters - physiology; ATP-binding protein; Bariatric Surgery - rehabilitation; Biochemistry; Biological and medical sciences; Blood; Cells, Cultured; CHO Cells; Cholesterol; Cholesterol - blood; Cholesterol - metabolism; Cholesteryl ester transfer protein; Cricetinae; Cricetulus; Endocrinopathies; Feeding. Feeding behavior; Female; Follow-Up Studies; Fundamental and applied biological sciences. Psychology; Gastrointestinal surgery; High density lipoprotein; Humans; Lipids; Medical sciences; Mice; Middle Aged; Obesity; Obesity, Morbid - blood; Obesity, Morbid - metabolism; Obesity, Morbid - surgery; Proteins; Rats; Scavenger receptors; Scavenger Receptors, Class B - genetics; Scavenger Receptors, Class B - physiology; Surgery; Vertebrates: anatomy and physiology, studies on body, several organs or systems; Vertebrates: endocrinology; Weight; Weight control; Weight Loss - physiology; Women; Young Adult; Human; Obesity; Nutrition; Nutrition disorder; Bariatric surgery; Weight loss; Lipids; Metabolic diseases; Hyperlipoproteinemia; Lipoprotein; Cholesterol; Hypercholesterolemia; Adult; Female; Dyslipemia; Woman; Cell; ABC transporter; Endocrinology; Nutritional status
• ISSN: 0021-972X; 1945-7197; 1945-7197
• DOI: 10.1210/jc.2010-2378

Aim:We tested the hypothesis that quantitative changes in high-density lipoprotein (HDL) particles weight loss induced by Roux-en-Y bypass (RYGBP) in morbidly obese subjects might be associated with improved functionality of these particles in the reverse cholesterol transport pathway.Methods and Results:Thirty-four morbidly obese women were recruited and followed up before and 6 months after RYGBP. After surgery, along with a major weight loss (−20%; P < 0.0001), we observed a significant increase in HDL mass concentration (+14%; P < 0.04), reflecting a specific increase in large HDL2 subfraction levels (+42%; P < 0.01), whereas those of HDL3 remained unchanged. Cholesterol ester transfer protein activity decreased significantly (−15%; P < 0.0001). Efflux capacity of total plasma increased significantly via both scavenger receptor class B type I (SR-BI) (+58%; P < 0.0001) and ATP binding cassette G1 (ABCG1) (+26%; P < 0.0001) pathways. Such enhanced capacity resulted from increased capacity of HDL2 particles to mediate cholesterol efflux through the SR-BI pathway (+56%, P < 0.001) and from the increase plasma level of cholesteryl ester-rich HDL2 particles for the ABCG1 pathway.Conclusion:RYGBP-induced weight loss results in improvement in atherogenic lipid profile including a shift toward a more cardioprotective HDL subfraction profile. In addition, our in vitro studies demonstrated an increased in plasma efflux capacity via both SR-BI and ABCG1 after surgery.