Persistence of hepatitis C virus RNA in established human hepatocellular carcinoma cell lines

• Published in: Journal of medical virology Vol. 48; no. 2; pp. 133 - 140
• Main Authors: Tsuboi, So, Nagamori, Seishi, Miyazaki, Masahiro, Mihara, Koichiro, Fukaya, Kenichi, Teruya, Kiichiro, Kosaka, Tsunenori, Tsuji, Takao, Namba, Masayoshi
• Format: Journal Article
• Language: English
• Published: New York John Wiley & Sons, Inc 01.02.1996; Wiley-Liss
• Subjects: Adolescent; Adult; Aged; Base Sequence; Biological and medical sciences; Carcinoma, Hepatocellular - pathology; Carcinoma, Hepatocellular - virology; Child; cultured human hepatoma; DNA, Viral; Female; Fundamental and applied biological sciences. Psychology; HCV; Hepacivirus - genetics; Hepacivirus - physiology; hepatitis C virus; Humans; Infant; Male; Microbiology; Middle Aged; Molecular Sequence Data; PCR; Polymerase Chain Reaction; Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains; RNA, Viral; Sequence Homology, Nucleic Acid; Tumor Cells, Cultured; Virology; Virus Integration; Virus Latency; Human; Carcinoma; Virus multiplication; Hepatic disease; Malignant tumor; In vitro; Virus; Persistence; Cell line; Liver cell carcinoma; Digestive diseases; Flaviviridae; Hepatitis C virus
• ISSN: 0146-6615; 1096-9071
• DOI: 10.1002/(SICI)1096-9071(199602)48:2<133::AID-JMV3>3.0.CO;2-A

The persistence of the viral RNA of hepatitis C virus (HCV) was examined in 13 hepatocellular carcinoma (HCC) and two hepatoblastoma cell lines by reverse transcription followed by the polymerase chain reaction (RT‐PCR). HCV RNA was detected in three HCC lines (JHH‐1, JHH‐4, and JHH‐6) and negative‐strand viral RNA was found in JHH‐4, indicating that there is a putative replicative intermediate of HCV in JHH‐4 cells. To rule out the possibility of contamination, the partial nucleotide sequences of HCV‐specific PCR products of these three cell lines were determined. The clone from JHH‐1 belonged to genotype 1 (1a or 1b), and the clones from JHH‐4 and JHH‐6 belonged to genotype 2b, but their sequences differed from each other. These cell lines may be useful for studies related to HCV. © 1996 Wiley‐Liss, Inc.