Developmental expression of Rab11, a small GTP-binding protein in Drosophila epithelia

• Published in: Genesis (New York, N.Y. : 2000) Vol. 47; no. 1; pp. 32 - 39
• Main Authors: Sasikumar, Satish, Roy, Jagat Kumar
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.01.2009
• Subjects: amnioserosa; Animals; dorsal closure; Drosophila; Drosophila - embryology; Drosophila - genetics; Drosophila - growth & development; Drosophila - metabolism; Embryo, Nonmammalian - embryology; Embryo, Nonmammalian - metabolism; epithelial morphogenesis; Epithelium - embryology; Epithelium - metabolism; Gene Expression Regulation, Developmental; Golgi Apparatus - metabolism; lateral epidermis; rab GTP-Binding Proteins - genetics; rab GTP-Binding Proteins - metabolism; Rab11; vesicular trafficking
• ISSN: 1526-954X; 1526-968X
• DOI: 10.1002/dvg.20441

Intracellular membrane trafficking regulates a wide variety of developmental processes, including cell and tissue morphogenesis. Here we report developmental expression of Drosophila Rab11, a small GTP‐binding protein, required for both endocytic recycling and exocytosis. Rab11 is expressed in the epithelial cell types of diverse lineages at all developmental stages, beginning from the cellular blastoderm in early embryos to adult primordia and adult tissues, like the columnar epithelia lining male ejaculatory bulb. A robust expression of Rab11 is seen both in the amnioserosa and in the lateral epidermis during embryonic dorsal closure, a morphogenetic event that involves spreading and fusion of the contra‐lateral sides of epidermis. Rab11 mutant embryos fail to display the characteristic morphological changes in these two epithelial tissues during dorsal closure, providing a strong basis to dissect the role of Rab11 in coordinated epithelial sheet movements. genesis 47:32–39, 2009. © 2008 Wiley‐Liss, Inc.