Simultaneous determination of carbohydrates and simmondsins in jojoba seed meal ( Simmondsia chinensis) by gas chromatography

• Published in: Journal of Chromatography A Vol. 977; no. 2; pp. 257 - 264
• Main Authors: Lein, Sabine, Van Boven, Maurits, Holser, Ron, Decuypere, Eddy, Flo, Gerda, Lievens, Sylvia, Cokelaere, Marnix
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 22.11.2002; Elsevier
• Subjects: Acetonitriles - analysis; Biological and medical sciences; Calibration; Carbohydrates; Carbohydrates - analysis; Caryophyllaceae - chemistry; Chromatography, Gas - methods; Cyclohexanes; Feed and pet food industries; Food industries; Fundamental and applied biological sciences. Psychology; Glucosides - analysis; Reproducibility of Results; Seeds - chemistry; Simmondsins; Carbohydrates; Simmondsins; Food analysis; Jojoba seed meal; Simmondsia chinensis; Chemical analysis; Nitrile; Sucrose; Jojoba seed; Silylation; Derivatization; Solvent extraction; Disaccharide; Oilseed cake; Sample preparation; Cyclitol; Dicotyledones; Angiospermae; Carbohydrate; Glycoside; Monocyclic compound; Feed; Quantitative analysis; Electron impact; Inositol; Aldose; Coupled method; Gas chromatography; Buxaceae; Analysis method; Quality control; Simultaneous measurement; Spermatophyta; Anorectic; Mass spectrometry
• ISSN: 0021-9673
• DOI: 10.1016/S0021-9673(02)01410-3

Separate methods for the analyses of soluble carbohydrates in different plants and simmondsins in jojoba seed meal are described. A reliable gas chromatographic procedure for the simultaneous quantification of d-pinitol, myo-inositol, sucrose, 5-α- d-galactopyranosyl- d-pinitol, 2-α- d-galactopyranosyl- d-pinitol, simmondsin, 4-demethylsimmondsin, 5-demethylsimmondsin and 4,5-didemethylsimmondsin as trimethylsilyl derivatives in jojoba seed meal has been developed. The study of different extraction mixtures allowed for the quantitative recovery of the 9 analytes by a mixture of methanol–water (80:20, v/v) in the concentration range between 0.1 and 4%. Comparison of the separation parameters on three different capillary stationary phases with MS detection allowed for the choice of the optimal gas chromatographic conditions for baseline separation of the analytes.