Simultaneous determination of carbohydrates and simmondsins in jojoba seed meal ( Simmondsia chinensis) by gas chromatography
Separate methods for the analyses of soluble carbohydrates in different plants and simmondsins in jojoba seed meal are described. A reliable gas chromatographic procedure for the simultaneous quantification of d-pinitol, myo-inositol, sucrose, 5-α- d-galactopyranosyl- d-pinitol, 2-α- d-galactopyrano...
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Published in | Journal of Chromatography A Vol. 977; no. 2; pp. 257 - 264 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Amsterdam
Elsevier B.V
22.11.2002
Elsevier |
Subjects | |
Online Access | Get full text |
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Summary: | Separate methods for the analyses of soluble carbohydrates in different plants and simmondsins in jojoba seed meal are described. A reliable gas chromatographic procedure for the simultaneous quantification of
d-pinitol,
myo-inositol, sucrose, 5-α-
d-galactopyranosyl-
d-pinitol, 2-α-
d-galactopyranosyl-
d-pinitol, simmondsin, 4-demethylsimmondsin, 5-demethylsimmondsin and 4,5-didemethylsimmondsin as trimethylsilyl derivatives in jojoba seed meal has been developed. The study of different extraction mixtures allowed for the quantitative recovery of the 9 analytes by a mixture of methanol–water (80:20, v/v) in the concentration range between 0.1 and 4%. Comparison of the separation parameters on three different capillary stationary phases with MS detection allowed for the choice of the optimal gas chromatographic conditions for baseline separation of the analytes. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0021-9673 |
DOI: | 10.1016/S0021-9673(02)01410-3 |