A novel solid support for synthesis of oligonucleotide 3′-phosphorothioate monoesters

• Published in: Bioorganic & medicinal chemistry letters Vol. 13; no. 2; pp. 281 - 284
• Main Authors: Cheruvallath, Zacharia S, Cole, Douglas L, Ravikumar, Vasulinga T
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01.01.2003; Elsevier
• Subjects: Ammonia - chemistry; Carbohydrates. Nucleosides and nucleotides; Chemistry; Electrophoresis, Capillary; Exact sciences and technology; Indicators and Reagents; Magnetic Resonance Spectroscopy; Nucleosides, nucleotides and oligonucleotides; Oligonucleotides - chemical synthesis; Organic chemistry; Organothiophosphorus Compounds - chemical synthesis; Preparations and properties; Immobilization; Nucleotide; Oligonucleotide; Solid phase; Reaction support; Organic thiophosphate; Chemical synthesis
• ISSN: 0960-894X; 1464-3405
• DOI: 10.1016/S0960-894X(02)00922-8

A new reagent immobilized on solid support allowing for solid-phase synthesis of oligonucleotides with a 3′-terminal phosphorothioate monoester is described. The support is compatible with phosphoramidite chemistry for automated oligonucleotide synthesis. Final deprotection with ammonia under standard conditions leads to oligonucleotide 3′-terminal phosphorothioate. A new reagent immobilized on solid support allowing for solid-phase synthesis of oligonucleotides with a 3′-terminal phosphorothioate monoester is described. The support is compatible with phosphoramidite chemistry for automated oligonucleotide synthesis. Final deprotection with ammonia under standard conditions leads to oligonucleotide 3′-terminal phosphorothioate monoester (3′-TPT).