Maternal Endotoxin Exposure Results in Abnormal Neuronal Architecture in the Newborn Rabbit

• Published in: Developmental neuroscience Vol. 35; no. 5; pp. 396 - 405
• Main Authors: Balakrishnan, Bindu, Dai, Hui, Janisse, James, Romero, Roberto, Kannan, Sujatha
• Format: Journal Article
• Language: English
• Published: Basel, Switzerland S. Karger AG 01.01.2013
• Subjects: Animals; Animals, Newborn; Brain - drug effects; Brain - pathology; Dendritic Spines - drug effects; Dendritic Spines - pathology; Endotoxins - toxicity; Female; Lipopolysaccharides - toxicity; Maternal Exposure; Nerve Net - drug effects; Nerve Net - pathology; Neurons - drug effects; Neurons - pathology; Original Paper; Pregnancy; Prenatal Exposure Delayed Effects - pathology; Rabbits; Thalamus - drug effects; Thalamus - pathology; Cerebral palsy; Maternal intrauterine inflammation; Perinatal brain injury; Dendritic spine; Neuronal injury; Thalamus; Dendrites
• ISSN: 0378-5866; 1421-9859
• DOI: 10.1159/000353156

Maternal intrauterine inflammation/infection is a potential risk factor for the development of neurologic disorders such as cerebral palsy (CP) in preterm and term infants. CP is associated with white matter and grey matter injury. In the current study, we used a rabbit model of CP in which pregnant rabbits are administered intrauterine injections of the endotoxin lipopolysaccharide. We then investigated the extent of neuronal damage in the newborn kit brain. We observed an overall decrease in the number of MAP2-stained neurons and an increase in Fluoro-Jade C-stained cells in the anterior thalamus of 1-day-old rabbit brain. We also observed an overall decrease in the number of branching points and spine density in the retrosplenial cortex, a major output region of the anterior thalamus that is involved in cognition and memory. The loss of spines and dendritic atrophy in the retrosplenial cortex may be caused by loss of presynaptic input from the thalamus. Our study indicates that the cognitive impairments seen in patients with CP may be related to the degeneration of neurons and abnormal arborization of the thalamic and cortical neurons.