Studies on Candidate Genes Related to Flowering Time in a Multiparent Population of Maize Derived from Tropical and Temperate Germplasm

• Published in: Plants (Basel) Vol. 13; no. 7; p. 1032
• Main Authors: Ran, Fengyun, Wang, Yizhu, Jiang, Fuyan, Yin, Xingfu, Bi, Yaqi, Shaw, Ranjan K, Fan, Xingming
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01.04.2024
• Subjects: Agricultural production; Agricultural research; Corn; Drought; Environmental aspects; estimated breeding value; Flowering; Genes; Genetic aspects; Genome-wide association studies; Genomes; Germplasm; Germplasm resources, Plant; GWAS; Haplotypes; Hybrids; maize flowering time; Molecular modelling; Physiological aspects; Plant breeding; Plant genetics; Plants, Flowering of; Pollination; Single-nucleotide polymorphism; Subpopulations; Temperate climates; tropical maize germplasm; Tropics; China; tropical maize germplasm; GWAS; maize flowering time; estimated breeding value
• ISSN: 2223-7747; 2223-7747
• DOI: 10.3390/plants13071032

A comprehensive study on maize flowering traits, focusing on the regulation of flowering time and the elucidation of molecular mechanisms underlying the genes controlling flowering, holds the potential to significantly enhance our understanding of the associated regulatory gene network. In this study, three tropical maize inbreds, CML384, CML171, and CML444, were used, along with a temperate maize variety, Shen137, as parental lines to cross with Ye107. The resulting F1s underwent seven consecutive generations of self-pollination through the single-seed descent (SSD) method to develop a multiparent population. To investigate the regulation of maize flowering time-related traits and to identify loci and candidate genes, a genome-wide association study (GWAS) was conducted. GWAS analysis identified 556 SNPs and 12 candidate genes that were significantly associated with flowering time-related traits. Additionally, an analysis of the effect of the estimated breeding values of the subpopulations on flowering time was conducted to further validate the findings of the present study. Collectively, this study offers valuable insights into novel candidate genes, contributing to an improved understanding of maize flowering time-related traits. This information holds practical significance for future maize breeding programs aimed at developing high-yielding hybrids.