Transcutaneous immunization with a 40-kDa outer membrane protein of Porphyromonas gingivalis induces specific antibodies which inhibit coaggregation by P. gingivalis

• Published in: Vaccine Vol. 23; no. 19; pp. 2513 - 2521
• Main Authors: Maeba, Satomi, Otake, Shigeo, Namikoshi, Jun, Shibata, Yasuko, Hayakawa, Mitsuo, Abiko, Yoshimitsu, Yamamoto, Masafumi
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 31.03.2005; Elsevier
• Subjects: 40-kDa outer membrane protein; Adjuvants, Immunologic - administration & dosage; Adjuvants, Immunologic - pharmacology; Administration, Cutaneous; Animals; Antibodies, Bacterial - analysis; Antibodies, Bacterial - blood; Applied microbiology; Bacterial Outer Membrane Proteins - administration & dosage; Bacterial Outer Membrane Proteins - immunology; Bacterial Vaccines - administration & dosage; Bacterial Vaccines - immunology; Bacteroidaceae Infections - prevention & control; Biological and medical sciences; Cholera Toxin - administration & dosage; Cholera Toxin - pharmacology; Fundamental and applied biological sciences. Psychology; Immunoglobulin A - blood; Immunoglobulin G - analysis; Mice; Mice, Inbred BALB C; Microbiology; Models, Animal; Molecular Weight; Periodontitis - prevention & control; Porphyromonas gingivalis; Porphyromonas gingivalis - immunology; Saliva - immunology; Transcutaneous immunization; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects); Porphyromonas gingivalis; 40-kDa outer membrane protein; Transcutaneous immunization; Immunization; Membrane protein; Antibody; Bacteria; Vaccine; External membrane; Bacteroidaceae; Percutaneous route
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/j.vaccine.2004.10.036

This study seeks to assess the potential of a 40-kDa outer membrane protein of Porphyromonas gingivalis (40k-OMP) as a transcutaneous vaccine against chronic periodontitis. Transcutaneous immunization (TCI) of mice with 40k-OMP alone elicited 40k-OMP-specific IgG antibody (Ab) responses in both serum and saliva. When administered with cholera toxin (CT) as adjuvant, TCI with 40k-OMP not only elevated IgG Abs as noted above, but also induced IgA responses in serum but not in saliva. Salivary IgG from mice given 40k-OMP alone or 40k-OMP plus CT showed higher binding levels to the 40k-OMP than did that of non-immunized mice. Ab-forming cell (AFC) analysis revealed high numbers of 40k-OMP-specific IgG AFCs in the spleen but low numbers in the salivary glands of mice given 40k-OMP alone or 40k-OMP plus CT. Since 40k-OMP-specific IgG inhibited the coaggregation of P. gingivalis vesicles and S. gordonii, TCI with 40k-OMP may be a useful tool in the quest to prevent P. gingivalis infection.