Selective determination of ammonium ions by high-speed ion-exclusion chromatography on a weakly basic anion-exchange resin column

• Published in: Journal of Chromatography A Vol. 997; no. 1; pp. 191 - 197
• Main Authors: Mori, Masanobu, Tanaka, Kazuhiko, Helaleh, Murad I.H., Xu, Qun, Ikedo, Mikaru, Ogura, Yutaka, Sato, Shinji, Hu, Wenzhi, Hasebe, Kiyoshi
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Amsterdam Elsevier B.V 16.05.2003; Elsevier
• Subjects: Ammonium ions; Analysis methods; Analytical chemistry; Anion Exchange Resins; Applied sciences; Calibration; Cations; Chemistry; Chromatographic methods and physical methods associated with chromatography; Chromatography, Ion Exchange - methods; Earth, ocean, space; Exact sciences and technology; External geophysics; Hydrogen-Ion Concentration; Indicators and Reagents; Inorganic cations; Meteorology; Natural water pollution; Other chromatographic methods; Pollution; Polymethacrylic Acids; Quaternary Ammonium Compounds - analysis; Sensitivity and Specificity; Tetrabutylammonium hydroxide; Water - analysis; Water in the atmosphere (humidity, clouds, evaporation, precipitation); Water Pollutants - analysis; Water treatment and pollution; Inorganic cations; Ammonium ions; Environmental analysis; Water analysis; Tetrabutylammonium hydroxide; Trace analysis; Methacrylate polymer; Ammonium; Chemical analysis; Phase composition; Anionic resin; Ion exclusion chromatography; Mobile phase; Inorganic cation; Ion chromatography; Conductometry; Surface water; Rain water; Water pollution; River water; Quantitative analysis
• ISSN: 0021-9673
• DOI: 10.1016/S0021-9673(03)00625-3

This paper describes an ion-exclusion chromatographic system for the rapid and selective determination of ammonium ion. The optimized ion-exclusion chromatographic system was established with a polymethacrylate-based weakly basic anion-exchange resin column (TSKgel DEAE-5PW) as the separation column, an aqueous solution containing 0.05 m M tetramethylammonium hydroxide (pH 9.10) as eluent with conductimetric detection for the analyte determination. Under the optimum chromatographic conditions, ammonium ion was determined within 2.3 min with a detection limit ( S/ N=3) better than 0.125 μ M. Ammonium ion in rain and river waters was precisely determined using this ion-exclusion chromatographic system.