Digital Image Processing for Rapid Analysis of Differentially Expressed Transcripts on High-Density cDNA Arrays

• Published in: BioTechniques Vol. 26; no. 6; pp. 1174 - 1179
• Main Authors: Adryan, Boris, Carlguth, Volker, Decker, Hans Jochen
• Format: Journal Article
• Language: English
• Published: Natick, MA Future Science Ltd 01.06.1999; Eaton
• Subjects: Autoradiography; Biological and medical sciences; Cloning, Molecular - methods; Color; Densitometry - methods; Diverse techniques; DNA, Complementary - analysis; Fundamental and applied biological sciences. Psychology; Gene Expression; General aspects; Image Processing, Computer-Assisted - methods; Mathematics in biology. Statistical analysis. Models. Metrology. Data processing in biology (general aspects); Molecular and cellular biology; Software; Transcription, Genetic; Computer science; Array; Autoradiography; Molecular hybridization; Analysis method; Complementary DNA; Software
• ISSN: 0736-6205; 1940-9818
• DOI: 10.2144/99266bc03

Usage of filter arrays is becoming increasingly attractive for many research laboratories involved in determination of gene-expression profiles. However, analysis of numerous spots, representing genes or partial gene sequences (ESTs), is still tedious work involving the ordered analysis of vast amounts of numerical tabular data. We present a rapid and efficient method for the visual identification of differentially expressed targets on high-density cDNA filter arrays using standard laboratory equipment and standard software, which is available for free. The method we introduce provides an inexpensive alternative, and no changes in the experimental set up are required. Our results were verified by densitometric analyses performed with an established system.