Exogenous Cytokinin 4PU-30 Modulates the Response of Wheat and Einkorn Seedlings to Ultraviolet B Radiation

• Published in: Plants (Basel) Vol. 13; no. 10; p. 1401
• Main Authors: Kirova, Elisaveta, Moskova, Irina, Manova, Vasilissa, Koycheva, Yana, Tsekova, Zoia, Borisova, Denitsa, Nikolov, Hristo, Dimitrov, Ventzeslav, Sergiev, Iskren, Kocheva, Konstantina
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01.05.2024
• Subjects: Abiotic stress; Analysis; antioxidant gene expression; Antioxidants; Cell division; Chlorophyll; Cytokinins; Enzymatic activity; Enzymes; flavonoids; Flowers & plants; Gene expression; Genes; Genotypes; Growth; Herbicides; Hydrogen peroxide; Influence; Leaves; Oxidative stress; phenolic compounds; phenylurea-cytokinin; Plant defenses; Plant growth; Plant resistance; Plant species; proline; Remote sensing; Salinity; Seedlings; Triticum monococcum; Ultraviolet radiation; Wheat; Bulgaria; antioxidant gene expression; proline; phenylurea-cytokinin; ROS; phenolic compounds; remote sensing; spectral reflectance; flavonoids; oxidative stress
• ISSN: 2223-7747; 2223-7747
• DOI: 10.3390/plants13101401

Abiotic stress is responsible for a significant reduction in crop plant productivity worldwide. Ultraviolet (UV) radiation is a natural component of sunlight and a permanent environmental stimulus. This study investigated the distinct responses of young wheat and einkorn plants to excessive UV-B radiation (180 min at λ 312 nm) following foliar pretreatment with 1 µM synthetic cytokinin 4PU-30. Results demonstrated that UV radiation significantly amplified hydrogen peroxide levels in both wheat and einkorn, with einkorn exhibiting a more pronounced increase compared to wheat. This elevation indicated the induction of oxidative stress by UV radiation in the two genotypes. Intensified antioxidant enzyme activities and the increased accumulation of typical stress markers and non-enzyme protectants were evidenced. Transcriptional activity of genes encoding the key antioxidant enzymes POX, GST, CAT, and SOD was also investigated to shed some light on their genetic regulation in both wheat and einkorn seedlings. Our results suggested a role for and genes in the UV-B tolerance of the two wheat species as well as a cytokinin-stimulated UV-B stress response in einkorn involving the upregulation of the tau subfamily gene . Based on all our findings, it could be concluded that 4PU-30 had the potential of alleviating oxidative stress by attenuating the symptoms of superfluous UV-B illumination in the two examined plant species.