Impacts of Mariculture on the Diversity of Bacterial Communities within Intertidal Sediments in the Northeast of China

• Published in: Microbial ecology Vol. 66; no. 4; pp. 861 - 870
• Main Authors: Li, Jialin, Li, Fuchao, Yu, Shuxian, Qin, Song, Wang, Guangyi
• Format: Journal Article
• Language: English
• Published: New York Springer Science + Business Media 01.11.2013; Springer US; Springer; Springer Nature B.V
• Subjects: Actinobacteria; Bacteria; Bacteria - classification; Bacteria - genetics; Bacteria - isolation & purification; Biodiversity; Biological and medical sciences; Biomedical and Life Sciences; China; Coastal zone; Communities; Community structure; Ecology; Ecosystem; ENVIRONMENTAL MICROBIOLOGY; Fish culture; Fisheries; Flavobacteria; Fundamental and applied biological sciences. Psychology; Geoecology/Natural Processes; Geologic Sediments - analysis; Geologic Sediments - microbiology; Libraries; Life Sciences; Mariculture; Marine; Marine aquaculture; Microbial Ecology; Microbiology; Molecular Sequence Data; Nature Conservation; Organic farming; Phylogenetics; Phylogeny; Planctomycetes; Seafood; Sediments; Silicon Dioxide - analysis; Sustainable agriculture; Sustainable communities; Water Quality/Water Pollution; Asia; China; INW, China, People's Rep., Hebei Prov., Qinhuangdao; INW, China, People's Rep., Liaoning Prov., Dalian; Bacterial Community; Terminal Restriction Fragment Length Polymorphism; Clone Library; Bacterial Community Composition; Bacterial Diversity; Environmental factor; Intertidal environment; Ecology; Sediments; Tides; Microbial community
• ISSN: 0095-3628; 1432-184X
• DOI: 10.1007/s00248-013-0272-6

Mariculture is one of the major seafood supplies worldwide and has caused serious environmental concerns on the coastal zone. Its rapid development has been shown to disrupt the sediment ecosystems and thus influence the benthic bacterial communities. Bacterial diversity and community structure within both adjacent farms and non-cultured zones intertidal sediments along the coasts of Qinhuangdao and Dalian, China, were investigated using full-length 16S rRNA gene-based T-RFLP analyses and clone library construction. Richness and Shannon—Wiener index were significantly increased at sites adjacent the mariculture farm with mean values of 29 and 2.97 from peak profiles of T-RFLP result. Clustering analyses suggested that impacts of mariculture on bacterial diversity of sediment were significantly larger than those resulted from temporal and spatial scales. Upon comparisons of RFLP patterns from 602 clones from libraries of the selected five samples, 137 OTUs were retrieved. Members of γ- and δ-Proteobacteria, Bacilli, Flavobacteria, and Actinobacteria were recorded in all libraries. In addition, γ-Proteobacteria were dominant in all samples (21.7∼45.0 %). Redundancy analysis revealed that the distribution of bacterial composition seemed to be determined by the variables of salinity, PO 4 3- -P, NH 4 + -N, and Chlorophyll a content. The phyla of γ-Proteobacteria, Clostridia, Flavobacteria, Bacilli, and Planctomycetes were principal components to contribute to the bacterial differences of clone libraries. Our finding demonstrated that these phyla could display variations of bacterial composition linked to environmental disturbance resulted from mariculture.