Molecular Detection of Bartonella bacilliformis in Lutzomyia maranonensis in Cajamarca, Peru: A New Potential Vector of Carrion's Disease in Peru?
Carrion's disease is a neglected, vector-borne illness that affects Colombia, Ecuador, and especially Peru. The phlebotomine sand flies and are the main illness vectors described, although other species may be implicated in endemic areas such as some northern Peruvian regions, in which Carrion&...
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Published in | The American journal of tropical medicine and hygiene Vol. 99; no. 5; pp. 1229 - 1233 |
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Main Authors | , , , , , , |
Format | Journal Article Publication |
Language | English |
Published |
United States
Institute of Tropical Medicine
01.01.2018
The American Society of Tropical Medicine and Hygiene |
Subjects | |
Online Access | Get full text |
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Summary: | Carrion's disease is a neglected, vector-borne illness that affects Colombia, Ecuador, and especially Peru. The phlebotomine sand flies
and
are the main illness vectors described, although other species may be implicated in endemic areas such as some northern Peruvian regions, in which Carrion's disease vector has not been established. The aim of this study was to evaluate the presence of
DNA in
from Cajamarca, northern Peru. This sand fly has not been defined as a vector yet. Centers for Disease Control and Prevention light traps were used to collect adult phlebotomine sand flies from 2007 to 2008 in the Cajamarca department. Female specimens were identified using morphological keys and were grouped into pools of five sand flies, taking into account district and sampling site (intradomicile or peridomicile). DNA was extracted, and then conventional and real-time polymerase chain reaction (RT-PCR) were performed to detect
and subsequently confirmed by sequencing. A total of 383 specimens of
species were analyzed. Two of 76 pools were positive for
by sequencing; all positives pools were from Querocotillo district. In addition,
spp. were identified in two pools of sand flies, which is an α-proteobacteria phylogenetically very close to
This study presents molecular evidence that suggests
is naturally infected by
in the Cajamarca department. Further research should determine if
is a vector and could transmit |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Authors’ addresses: Gabriela M. Ulloa, Centro de Investigación e Innovación, Facultad de Ciencias de la Salud, Universidad Peruana de Ciencias Aplicadas, Lima, Peru, and Emerge, Emerging Diseases and Climate Change Research Unit, School of Public Health and Administration, Universidad Peruana Cayetano Heredia, Lima, Peru, E-mail: gulloau92@gmail.com. Fernando Vásquez-Achaya and Juana del Valle Mendoza, Centro de Investigación e Innovación, Facultad de Ciencias de la Salud, Universidad Peruana de Ciencias Aplicadas, Lima, Peru, E-mails: fernando.vasquez.achaya@gmail.com and juana.delvalle@upc.edu.pe. Cláudia Gomes and Joaquim Ruiz, ISGlobal, Hospital Clínic—Universitat de Barcelona, Barcelona, Spain, E-mails: claudiasofiapgomes@gmail.com and joruiz.trabajo@gmail.com. Luis J. del Valle, Departament d’Enginyeria Agroalimentària i Biotecnologia, Universitat Politècnica de Catalunya, Barcelona, Spain, E-mail: luis.javier.del.valle@upc.edu. Maria J. Pons, Microbiología molecular y Genómica bacteriana, Universidad Cientifica del Sur, Lima, Peru, E-mail: ma.pons.cas@gmail.com. Financial support: This work was supported by the Royal Society of Tropical Medicine and Hygiene (GR000589), Programa Nacional de Innovación para la Competitividad y Productividad (Innóvate Perú)-contract 116-PNICP-PIAP-2015. J. R. had a fellowship from the program I3SNS of the ISCIII (grant number: CES11/012); C. G. had a predoctoral grant from the ISCIII (FI12/00561). |
ISSN: | 0002-9637 1476-1645 |
DOI: | 10.4269/ajtmh.18-0520 |