Characterization of thraustochytrid-specific sterol O -acyltransferase: modification of DGAT2-like enzyme to increase the sterol production in Aurantiochytrium limacinum mh0186

Since the global market for sterols and vitamin D are grown with a high compound annual growth rate, a sustainable source of these compounds is required to keep up with the increasing demand. Thraustochytrid is a marine oleaginous microorganism that can synthesize several sterols, which are stored a...

Full description

Saved in:
Bibliographic Details
Published inApplied and environmental microbiology Vol. 89; no. 11; p. e0100123
Main Authors Ishibashi, Yohei, Sadamitsu, Shohei, Fukahori, Yoshitomo, Yamamoto, Yuki, Tanogashira, Rin, Watanabe, Takashi, Hayashi, Masahiro, Ito, Makoto, Okino, Nozomu
Format Journal Article
LanguageEnglish
Published United States American Society for Microbiology 29.11.2023
Subjects
Biotechnology
Cholesterol
Deletion
Diacylglycerol O-acyltransferase
Diacylglycerol O-Acyltransferase - genetics
Diglycerides
Droplets
Endoplasmic reticulum
Intermediates
Lipid Droplets
Lipids
Localization
Marine microorganisms
Microorganisms
Polyunsaturated fatty acids
Sterol O-Acyltransferase
Sterols
Synthesis
Transmembrane domains
Triglycerides
Vitamin D
DGAT
sterol ester
sterols
sterol o-acyltransferase
thraustochytrid
diacylglycerol
Online AccessGet full text

Cover

More Information
Summary:Since the global market for sterols and vitamin D are grown with a high compound annual growth rate, a sustainable source of these compounds is required to keep up with the increasing demand. Thraustochytrid is a marine oleaginous microorganism that can synthesize several sterols, which are stored as SE in lipid droplets. DGAT2C is an unconventional SE synthase specific to thraustochytrids. Although the primary structure of DGAT2C shows high similarities with that of DGAT, DGAT2C utilizes sterol as an acceptor substrate instead of diacylglycerol. In this study, we examined more detailed enzymatic properties, intracellular localization, and structure-activity relationship of DGAT2C. Furthermore, we successfully developed a method to increase sterol and provitamin D3 productivity of thraustochytrid by more than threefold in the process of elucidating the function of the DGAT2C-specific N-terminal region. Our findings could lead to sustainable sterol and vitamin D production using thraustochytrid.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
The authors declare no conflict of interest.
ISSN:0099-2240
1098-5336
DOI:10.1128/aem.01001-23