Characterization of a First Domain of Human High Glycine-Tyrosine and High Sulfur Keratin-associated Protein (KAP) Genes on Chromosome 21q22.1

Analysis of the EBI/GeneBank TM data base using non-human hair keratin-associated protein (KAP) cDNA sequences as a query resulted in the identification of a first domain of high glycine-tyrosine and high sulfur KAP genes located on human chromosome 21q22.1. This domain, present on the DNA accession...

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Published inThe Journal of biological chemistry Vol. 277; no. 50; pp. 48993 - 49002
Main Authors Rogers, Michael A, Langbein, Lutz, Winter, Hermelita, Ehmann, Claudia, Praetzel, Silke, Schweizer, Jürgen
Format Journal Article
LanguageEnglish
Published United States American Society for Biochemistry and Molecular Biology 13.12.2002
Subjects
Amino Acid Sequence
Base Sequence
Chromosome Mapping
Chromosomes, Human, Pair 21
DNA Primers
Gene Expression
Glycine - metabolism
Hair - metabolism
Humans
Keratins - chemistry
Keratins - genetics
Keratins - metabolism
Molecular Sequence Data
Sequence Homology, Amino Acid
Tyrosine - metabolism
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Summary:Analysis of the EBI/GeneBank TM data base using non-human hair keratin-associated protein (KAP) cDNA sequences as a query resulted in the identification of a first domain of high glycine-tyrosine and high sulfur KAP genes located on human chromosome 21q22.1. This domain, present on the DNA accession numbers AP001078 and AP001709 , was ∼535 kb in size and contained 17 high glycine-tyrosine and 7 high sulfur KAP genes, as well as 9 KAP pseudogenes. Based on amino acid sequence comparisons of the encoded proteins, the KAP genes could be divided into seven high glycine-tyrosine gene families (KAP6–KAP8, and KAP19–KAP22) and four high sulfur gene families (KAP11, KAP13, KAP15, and KAP23). The high glycine-tyrosine genes described here appear to represent the complete set of this type of KAP genes present in the human genome. Both systematic cDNA isolation studies from an arrayed scalp cDNA library and in situ hybridization expression studies of all of the KAP genes identified in the 21q22.1 region revealed varying degrees and regions of expression of 11 members of the high tyrosine-glycine genes and 6 members of the high sulfur KAP genes in the hair forming compartment.
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ISSN:0021-9258
1083-351X
DOI:10.1074/jbc.M206422200