EFFECT OF CYCLOSPORINE ON HUMAN SPERM MOTILITY IN VITRO

• Published in: Archives of andrology Vol. 43; no. 3; pp. 215 - 220
• Main Authors: Misro, M.M, Chaki, S.P, Srinivas, M., Chaube, S.K
• Format: Journal Article
• Language: English
• Published: Philadelphia, PA Informa UK Ltd 1999; Taylor & Francis
• Subjects: Biological and medical sciences; Cell physiology; Cyclosporine - pharmacology; Cyclosporine;Incubation;Motility;Sperm; Fundamental and applied biological sciences. Psychology; Humans; Immunosuppressive Agents - pharmacology; In Vitro Techniques; Male; Molecular and cellular biology; Motility and taxis; Sperm Motility - drug effects; Human; Culture medium; Spermatozoa; Motility; Enzyme; Ciclosporin; Serum albumin; Immunosuppressive agent; Oxidoreductases; Germinal cell; In vitro; L-Lactate dehydrogenase
• ISSN: 0955-3002; 0148-5016; 1362-3095; 1521-0375
• DOI: 10.1080/014850199262526

Cyclosporine affects motility and viability of human sperm when incubated together in vitro. Sperm motility was almost reduced to nil following 10 min of incubation with cyclosporine at a concentration of 1 mg/mL. However, 200 mug/mL of the drug has no effect on motility and viability when tested for up to 60 min under standard laboratory conditions. Cyclosporine effect on sperm was both dose and time dependent. Sperm sensitivity and susceptibility to cyclosporine even to lower doses increased significantly following withdrawal of bovine serum albumin from the incubating medium. Compared to untreated controls, lactate dehydrogenase was estimated higher by more than 2 to 4 times in the spermfree incubating media, suggesting an altered membrane porosity in the affected spermatozoa.