Recombinant bromelain production in Escherichia coli: process optimization in shake flask culture by response surface methodology

• Published in: AMB Express Vol. 2; no. 1; p. 12
• Main Authors: Muntari, Bala, Amid, Azura, Mel, Maizirwan, Jami, Mohammed S, Salleh, Hamzah M
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 15.02.2012; Springer Nature B.V; Springer
• Subjects: Biomedical and Life Sciences; Biotechnology; Life Sciences; Microbial Genetics and Genomics; Microbiology; Original; face centered central composite design; induction; bromelain; BL21-AI
• ISSN: 2191-0855; 2191-0855
• DOI: 10.1186/2191-0855-2-12

Bromelain, a cysteine protease with various therapeutic and industrial applications, was expressed in Escherichia coli , BL21-AI clone, under different cultivation conditions (post-induction temperature, L-arabinose concentration and post-induction period). The optimized conditions by response surface methodology using face centered central composite design were 0.2% (w/v) L-arabinose, 8 hr and 25°C. The analysis of variance coupled with larger value of R 2 (0.989) showed that the quadratic model used for the prediction was highly significant ( p < 0.05). Under the optimized conditions, the model produced bromelain activity of 9.2 U/mg while validation experiments gave bromelain activity of 9.6 ± 0.02 U/mg at 0.15% (w/v) L-arabinose, 8 hr and 27°C. This study had innovatively developed cultivation conditions for better production of recombinant bromelain in shake flask culture.