Enzymatic production of D-glu from L-glu by Lactobacillus brevis ATCC 8287

• Published in: Bioscience, biotechnology, and biochemistry Vol. 57; no. 9; pp. 1499 - 1502
• Main Authors: Yagasaki, Makoto, Ozaki, Akio, Hashimoto, Yukio
• Format: Journal Article
• Language: English
• Published: Tokyo Taylor & Francis 1993; Japan Society for Bioscience Biotechnology and Agrochemistry; Oxford University Press
• Subjects: acide glutamique; acido glutamico; Bacteriology; Bioconversions. Hemisynthesis; Biological and medical sciences; Biotechnology; Fundamental and applied biological sciences. Psychology; glutamate decarboxylase; glutamato decarboxilasa; glutamic acid; isomerasas; isomerase; isomerases; isomerisation; isomerizacion; isomerization; lactobacillus; Lactobacillus brevis; Metabolism. Enzymes; Methods. Procedures. Technologies; Microbiology; Glutamate racemase; D stereoisomer; Enzyme; Racemization; Lyases; Glutamate decarboxylase; Carbon-carbon lyases; Isomerases; Enzymatic activity; Lactobacillus brevis; Carboxy-lyases; Biotransformation; L stereoisomer; Bacteria; Lactobacillaceae; Physicochemical properties
• ISSN: 0916-8451; 1347-6947
• DOI: 10.1271/bbb.57.1499

Enzymatic production of D-Glu was investigated by the succesive reactions of a glutamate racemase (EC 5.1.1.3) and a glutamate decarboxylase (EC 4.1.1.15) on L-Glu.Lactobacillus brevis ATCC8287 was chosen as a source of glutamate racemase. This strain produced a glutamate decarboxylase simultaneously. The glutamate racemase activity in the cell free extracts was 0.035 units/mg protein. The enzyme kept its activity even at 500 Mm of L-Glu (74g/liter). The optimum pHs of the racemase and the decarboxylase were at around 8.5 and below 4.0, respectively. Both enzymes had no activity at the optimum pH for the other enzyme. L-Glu was racemized first by the glutamate racemase at pH 8.5, then the pH was shifted to 4.0 at which L-Glu was decarboxylated by the glutamate decarboxylase. Starting from 100 g/liter of L-Glu, 50 g/liter of D-Glu was produced and no L-Glu remained in the reaction mixture.