Real-time detection of l-ascorbic acid and hydrogen peroxide in crude food samples employing a reversed sequential differential measuring technique of the SIRE-technology based biosensor

• Published in: Biosensors & bioelectronics Vol. 16; no. 6; pp. 363 - 369
• Main Authors: Kriz, K., Anderlund, M., Kriz, D.
• Format: Journal Article
• Language: English
• Published: Lausanne Elsevier B.V 01.08.2001; Elsevier Science
• Subjects: Ascorbate Oxidase - analysis; Ascorbic acid; Ascorbic Acid - analysis; Biological and medical sciences; Biosensing Techniques - methods; Biosensor; Biosensors; Biotechnology; Calibration; Catalase - analysis; Differential measurement; Edible Grain - chemistry; Food Analysis - methods; Food industries; Fruit - chemistry; Fundamental and applied biological sciences. Psychology; General aspects; Hydrogen peroxide; Hydrogen Peroxide - analysis; Methods of analysis, processing and quality control, regulation, standards; Methods. Procedures. Technologies; SIRE; Various methods and equipments; Differential measurement; Ascorbic acid; Hydrogen peroxide; Biosensor; SIRE; Performance evaluation; Enzyme; Foodstuff; Real time; Hydrogen Peroxides; Analysis method; Peroxidases; Catalase; L-Ascorbate oxidase; Oxidoreductases; Quantitative analysis; Flow injection
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/S0956-5663(01)00151-8

Detection of the common electrochemical interferents, ascorbic acid and hydrogen peroxide, using a SIRE ( Sensors based on Injection of the Recognition Element) technology based biosensor in reverse mode operation is reported. The differential measuring principle employed in the SIRE biosensor during operation in reverse mode is such that the sample is measured first in the presence of enzyme (yielding matrix signal only), and then measured again in the absence of enzyme (yielding signal from matrix+analyte). Subtraction of the signal obtained in the presence of enzyme from the signal obtained in the absence of enzyme gives a specific signal for the analyte only and correlates directly to its concentration in solution. The linear range for the determination of ascorbic acid and hydrogen peroxide was 0–3 mM and 0–2 mM, respectively, with an enzyme concentration of 25 U ascorbate oxidase/ml and 1000 U catalase/ml. The reproducibility was 5% for ascorbic acid (R.S.D. n=15) and 10% for hydrogen peroxide (R.S.D. n=18). The cost per measurement was 0.28 USD for ascorbic acid analysis and 0.0008 USD for hydrogen peroxide analysis. The degradation of ascorbic acid in cereal was followed in real-time, as was the stabilization of low pH on the degradation process.