Dissecting Human Cytomegalovirus Gene Function and Capsid Maturation by Ribozyme Targeting and Electron Cryomicroscopy

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 102; no. 20; pp. 7103 - 7108
• Main Authors: Yu, Xuekui, Trang, Phong, Shah, Sanket, Atanasov, Ivo, Kim, Yong-Hwan, Bai, Yong, Zhou, Z. Hong, Liu, Fenyong, Altman, Sidney
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 17.05.2005; National Acad Sciences
• Subjects: Application programming interfaces; Biochemistry; Biological Sciences; Blotting, Northern; Blotting, Western; Capsid; Capsid Proteins - metabolism; Cell lines; Cells, Cultured; Cryoelectron Microscopy; Cytomegalovirus - genetics; Cytomegalovirus - metabolism; Cytomegalovirus - physiology; DNA; DNA Primers; Electron microscopes; Endopeptidases - metabolism; Gene Expression; Genes; Herpesviridae; Human cytomegalovirus; Humans; Infections; Messenger RNA; Proteins; Ribonuclease P - metabolism; RNA; RNA, Catalytic - metabolism; Scaffolds; Viral Proteins - metabolism; Virus Replication - physiology; Viruses
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.0408826102

Human CMV (HCMV) is the leading viral cause of birth defects and causes one of the most common opportunistic infections among transplant recipients and AIDS patients. Cleavage of internal scaffolding proteins by the viral protease (Pr) occurs during HCMV capsid assembly. To gain insight into the mechanism of HCMV capsid maturation and the roles of the Pr in viral replication, an RNase P ribozyme was engineered to target the Pr mRNA and down-regulate its expression by >99%, generating premature Pr-minus capsids. Furthermore, scaffolding protein processing and DNA encapsidation were inhibited by 99%, and viral growth was reduced by 10,000-fold. 3D structural comparison of the Pr-minus and wild-type B capsids by electron cryomicroscopy, at an unprecedented 12.5-Å resolution, unexpectedly revealed that the structures are identical in their overall shape and organization. However, the Pr-minus capsid contains tenuous connections between the scaffold and the capsid shell, whereas the wild-type B capsid has extra densities in its core that may represent the viral Pr. Our findings indicate that cleavage of the scaffolding protein is not associated with the morphological changes that occur during capsid maturation. Instead, the protease appears to be required for DNA encapsidation and the subsequent maturation steps leading to infectious progeny. These results therefore provide key insights into an essential step of HCMV infection using an RNase P ribozyme-based inhibition strategy.