CD86 molecule is a specific marker for canine monocyte-derived dendritic cells

• Published in: Veterinary Immunology and Immunopathology Vol. 109; no. 1; pp. 167 - 176
• Main Authors: Bonnefont-Rebeix, Catherine, de Carvalho, Camila Miranda, Bernaud, Janine, Chabanne, Luc, Marchal, Thierry, Rigal, Dominique
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15.01.2006
• Subjects: Animals; B7-2 Antigen - immunology; Biomarkers; Canine monocyte-derived dendritic cells; CD86; Cell Proliferation; Dendritic Cells - cytology; Dendritic Cells - immunology; Dog; Dogs - immunology; Female; Flow Cytometry - veterinary; Granulocyte-Macrophage Colony-Stimulating Factor - immunology; Histocompatibility Antigens Class II - immunology; Interleukin-4 - immunology; Kinetics; Lymphocyte Activation - immunology; Lymphocyte Culture Test, Mixed - veterinary; Male; Monocytes - cytology; Monocytes - immunology; PBS; CD86; cpm; PHA; Canine monocyte-derived dendritic cells; FACS; PBMC; LPS; mAb; MFI; MLR; cMo-DC; Dog; ConA; DC
• ISSN: 0165-2427; 1873-2534; 1365-2567
• DOI: 10.1016/j.vetimm.2005.08.027

In this study, canine monocyte-derived dendritic cells (cMo-DC) were produced in presence of canine GM-CSF (cGM-CSF) and canine IL-4 (cIL-4), and they were characterized by their dendritic morphology, MLR functionality and phenotype. We noticed that cMo-DC were labelled with three anti-human CD86 (FUN-1, BU63 and IT2.2 clones), whereas resting and activated lymphocytes or monocytes were not stained. CD86 expression was induced by cIL-4 and was up-regulated during the differentiation of the cMo-DC, with a maximum at day 7. Furthermore, cMo-DC were very potent even in low numbers as stimulator cells in allogeneic MLR, and BU63 mAb was able to completely block the cMo-DC-induced proliferation in MLR. We also observed that cMo-DC highly expressed MHC Class II and CD32, but we failed to determine their maturation state since the lack of commercially available canine markers. Moreover, cMo-DC contained cytoplasmic periodic microstructures, potentially new ultrastructural markers of canine DC recently described. In conclusion, this work demonstrates that the CD86 costimulatory marker is now usable for a better characterization of in vitro canine DC.