Metabolic syndrome and ADRB3 gene polymorphism in severely obese patients from South Italy

• Published in: European journal of clinical nutrition Vol. 61; no. 10; pp. 1213 - 1219
• Main Authors: Bracale, R, Pasanisi, F, Labruna, G, Finelli, C, Nardelli, C, Buono, P, Salvatori, G, Sacchetti, L, Contaldo, F, Oriani, G
• Format: Journal Article
• Language: English
• Published: Basingstoke Nature Publishing 01.10.2007; Nature Publishing Group
• Subjects: Adrenergic receptors; Adult; age; Age Factors; alleles; beta-adrenergic receptors; Biological and medical sciences; blood glucose; Blood Glucose - analysis; blood lipids; Blood pressure; Body Mass Index; Body size; Cross-Sectional Studies; Deoxyribonucleic acid; Diabetes mellitus; DNA; Female; gender differences; Gene Frequency; Gene polymorphism; genetic polymorphism; Genetic Predisposition to Disease; Genomics; Genotype; Genotype & phenotype; Homeostasis; hormone receptors; Humans; Insulin; Insulin - blood; Insulin resistance; Italy - epidemiology; Leptin; Leptin - blood; Leukocytes; Leukocytes - metabolism; Linear Models; Lipids; Lipids - blood; Male; Medical sciences; Men; Metabolic diseases; Metabolic disorders; Metabolic syndrome; Metabolic Syndrome - epidemiology; Metabolic Syndrome - genetics; Miscellaneous; Mutation; Obesity; Obesity, Morbid - blood; Obesity, Morbid - complications; Obesity, Morbid - genetics; Other metabolic disorders; Polymorphism; Polymorphism, Genetic; prevalence; Receptors (physiology); Receptors, Adrenergic, beta-3 - genetics; Sex Factors; women; Italy; Europe; Endocrinopathy; Human; Obesity; Nutrition; Nutrition disorder; South; Metabolic diseases; Cardiovascular disease; Patient; Metabolic syndrome; Gene; ADRB3 polymorphism; Genetics; Severe; severe obesity; Nutritional status; Polymorphism
• ISSN: 0954-3007; 1476-5640
• DOI: 10.1038/sj.ejcn.1602640

Objective: To evaluate the prevalence of β ₃-adrenergic receptor (ADRB3) Trp64Arg polymorphism and its relationship with the metabolic syndrome in severe obesity. Design: Cross-sectional outpatients study. Patients and methods: In 265 (100 men) severely obese non-diabetic subjects and 78 (25 men) healthy volunteers, genomic DNA was isolated from peripheral leukocytes. In obese patients, plasma concentrations of leptin, lipids, glucose and insulin, the homeostasis model assessment index and blood pressure have been measured. The Trp64Arg mutation was identified with the real-time TaqMan method. Results: Neither genotype distribution nor allele frequency differed between the two groups. The metabolic syndrome prevalence was 59% in obese subjects, and was higher in men than in women (65 vs 55%: P=0.03). The body mass index (BMI) was related to age tertiles (β=0.08; P<0.001; multiple linear regression) in Trp64Arg-positive obese subjects. Conclusion: We confirm the high prevalence of the metabolic syndrome among severely obese subjects. ADRB3 polymorphism was significantly related to insulin resistance only in obese male subjects. Moreover, increased BMI was related to age in obese subjects with the ADRB3 polymorphism.