Cis-12-Oxo-Phytodienoic Acid Stimulates Rice Defense Response to a Piercing-Sucking Insect

• Published in: Molecular plant Vol. 7; no. 11; pp. 1683 - 1692
• Main Authors: Guo, Hui-Min, Li, Hai-Chao, Zhou, Shi-Rong, Xue, Hong-Wei, Miao, Xue-Xia
• Format: Journal Article
• Language: English
• Published: England Elsevier Inc 01.11.2014
• Subjects: allene oxide cyclase; Animals; brown planthopper; cis-12-oxo-phytodienoic acid; Cyclopentanes; Fatty Acids, Unsaturated - physiology; Gene Expression Regulation, Plant; Hemiptera - physiology; Herbivory; Intramolecular Oxidoreductases - genetics; Intramolecular Oxidoreductases - metabolism; jasmonic acid; OPDA reductase 3; Oryza - enzymology; Oryza - genetics; Oryza - physiology; Oxidoreductases Acting on CH-CH Group Donors - genetics; Oxidoreductases Acting on CH-CH Group Donors - metabolism; Oxylipins; Plant Growth Regulators - physiology; Plant Proteins - genetics; Plant Proteins - metabolism; Plants, Genetically Modified - enzymology; Plants, Genetically Modified - physiology; 刺吸式害虫; 微阵列分析; 抗性相关基因; 抗褐飞虱; 植物防御反应; 氧代; 水稻害虫; 酸刺; brown planthopper; cis-12-oxo-phytodienoic acid; OPDA reductase 3; allene oxide cyclase; jasmonic acid
• ISSN: 1674-2052; 1752-9867
• DOI: 10.1093/mp/ssu098

The brown planthopper （BPH, Nilaparvata lugens） is a destructive, monophagous, piercing-sucking insect pest of rice. Previous studies indicated that jasmonic acid （JA） positively regulates rice defense against chewing insect pests but negatively regulates it against the piercing-sucking insect of BPH. We here demonstrated that overexpression of allene oxide cyclase （AOC） but not OPR3 （cis-12-oxo-phytodienoic acid （OPDA） reductase 3, an enzyme adjacent to AOC in the JA synthetic pathway） significantly increased rice resistance to BPH, mainly by reducing the feeding activity and survival rate. Further anal- ysis revealed that plant response to BPH under AOC overexpression was independent of the JA pathway and that significantly higher OPDA levels stimulated rice resistance to BPH. Microarray analysis identified multiple candidate resistance-related genes underAOCoverexpression. OPDA treatment stimulated the resistance of radish seedlings to green peach aphid Myzus persicae, another piercing-sucking insect. These results imply that rice resistance to chewing insects and to sucking insects can be enhanced simultaneously through AOC-mediated increases of JA and OPDA and provide direct evidence of the potential application of OPDA in stimulating plant defense responses to piercing-sucking insect pests in agriculture.