The effects of uncoupling protein-1 genotype on lipoprotein cholesterol level in Korean obese subjects

• Published in: Metabolism, clinical and experimental Vol. 53; no. 8; pp. 1054 - 1059
• Main Authors: Oh, Hyun Hee, Kim, Kil Soo, Choi, Sun Mi, Yang, Hyun Sung, Yoon, Yoosik
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 01.08.2004; Elsevier
• Subjects: Adult; Biological and medical sciences; Blood Glucose - metabolism; Blood Pressure - physiology; Body Composition - physiology; Body Mass Index; Body Weight - physiology; Carrier Proteins - genetics; Cholesterol - blood; Cholesterol, HDL - blood; Cholesterol, LDL - blood; Female; Gene Frequency; Genotype; Humans; Hypercholesterolemia - blood; Hypercholesterolemia - epidemiology; Hypercholesterolemia - genetics; Ion Channels; Korea - epidemiology; Lipoproteins - blood; Liver Function Tests; Logistic Models; Male; Medical sciences; Membrane Proteins - genetics; Metabolic diseases; Mitochondrial Proteins; Obesity; Obesity - blood; Obesity - epidemiology; Obesity - genetics; Polymorphism, Genetic - genetics; Polymorphism, Genetic - physiology; Uncoupling Protein 1; Human; Obesity; Uncoupling protein; Pathogenesis; Size; Nutrition disorder; Genotype; Lipids; Body composition; Hip; Weight; Blood plasma; Polymerase chain reaction; Body mass index; Metabolic disorder; Restriction fragment length polymorphism; Thermogenesis; Gene; Cholesterol HDL; Height; Ratio; Hemodynamics; Lipoprotein HDL; Nutritional status
• ISSN: 0026-0495; 1532-8600
• DOI: 10.1016/j.metabol.2004.02.014

Uncoupling protein-1 (UCP-1) plays a major role in thermogenesis, and has been implicated in the pathogenesis of obesity and metabolic disorders. The purpose of this study was to estimate the effects of A-3826G polymorphism of the UCP-1 gene on the plasma lipid profiles in 190 Korean obese subjects with a body mass index (BMI) more than 30 kg/m 2. Height, weight, BMI, wait-to-hip ratio (WHR), obesity index, and body composition were measured and genotype of UCP-1 was analyzed by polymerase chain reaction (PCR) restriction fragment length polymorphism (RFLP) method. Serum concentrations of fasting glucose, total cholesterol, high-density lipoprotein (HDL) cholesterol, and triglyceride were measured. The frequencies of UCP-1 genotypes were AA type, 22.1%; AG type, 53.7%; and GG type, 24.2%; and the frequency of G allele was 0.51. Among many parameters, diastolic blood pressure (DBP) ( P = .023) and low-density lipoprotein (LDL) cholesterol ( P = .011) were significantly higher in AG and GG types compared with AA type, whereas HDL cholesterol was significantly lower in GG type compared with other types ( P < .05). Atherogenic index was significantly higher in GG type compared with AA type ( P = 0.027). LDL-to-HDL cholesterol ratio was significantly increased in the order of AA < AG < GG types ( P = .001). When the subjects were divided into a normal group and a hyper-LDL cholesterolemia group by LDL cholesterol level of 3.626 mmol/L (140 mg/dL), the frequency of hyper-LDL cholesterolemia was significantly higher in GG type compared with other types by Fisher’s exact (chi-square) test ( P = .05). When logistic regression analysis was conducted to find the risk factors of hyper-LDL cholesterolemia, the odds ratio was 4.115 ( P = .03) for GG type of UCP-1 gene. These results suggest that the GG type of the UCP-1 gene has a strong association with increased LDL cholesterol level and might be a significant risk factor for hyper-LDL cholesterolemia among Korean obese subjects.