Functional Characterization of ShK Domain-Containing Protein in the Plant-Parasitic Nematode Bursaphelenchus xylophilus

ShK domain-containing proteins are peptides found in different parasitic and venomous organisms. From a previous transcriptomic dataset from , a plant-parasitic nematode that infects forest tree species, we identified 96 transcripts potentially as ShK domain-containing proteins with unknown function...

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Published inPlants (Basel) Vol. 13; no. 3; p. 404
Main Authors Mendonça, Madalena, Vicente, Cláudia S L, Espada, Margarida
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 01.01.2024
Subjects
Bursaphelenchus xylophilus
Catalase
Environmental aspects
Epidermal growth factor
Genes
Genetic aspects
Genomes
Genomics
Host plants
Hybridization
Identification and classification
Infections
molecular plant–nematode interaction
Nematodes
Oxidative stress
Parasitism
Peptides
Physiological aspects
pinewood nematode
Plant nematodes
Plant parasites
Plant species
Proteins
Reactive oxygen species
Transcription factors
Transcriptomics
Portugal
parasitism
pinewood nematode
oxidative stress
molecular plant–nematode interaction
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Summary:ShK domain-containing proteins are peptides found in different parasitic and venomous organisms. From a previous transcriptomic dataset from , a plant-parasitic nematode that infects forest tree species, we identified 96 transcripts potentially as ShK domain-containing proteins with unknown function in the nematode genome. This study aimed to characterize and explore the functional role of genes encoding ShK domain-containing proteins in biology. We selected and functionally analyzed nine candidate genes that are putatively specific to . In situ hybridization revealed expression of one ShK in the pharyngeal gland cells, suggesting their delivery into host cells. Most of the transcripts are highly expressed during infection and showed a significant upregulation in response to peroxide products compared to the nematode catalase enzymes. We reported, for the first time, the potential involvement of ShK domain genes in oxidative stress, suggesting that these proteins may have an important role in protecting or modulating the reactive oxygen species (ROS) activity of the host plant during parasitism.
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ISSN:2223-7747
2223-7747
DOI:10.3390/plants13030404