Evidence of zein-bound indoleacetic acid using gas chromatography-selected ion monitoring-mass spectrometry analysis and immunogold labeling

Commercial zein was base-hydrolyzed and purified extracts were subjected to gas chromatography-selected ion monitoring-mass spectrometry analysis. Indoleacetic acid (IAA) was shown to be released from this storage protein of corn (Zea mays). Isotope dilution using [13C6]IAA as an internal standard r...

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Bibliographic Details
Published inPlant physiology (Bethesda) Vol. 96; no. 4; pp. 1070 - 1075
Main Authors Leverone, L.A. (University of Cincinnati, Cincinnati, OH), Kossenjans, W, Jayasimihulu, K, Caruso, J.L
Format Journal Article
LanguageEnglish
Published United States American Society of Plant Physiologists 01.08.1991
Subjects
ACIDE INDOLACETIQUE
ACIDO INDOLACETICO
ANATOMIA DE LA PLANTA
ANATOMIE VEGETALE
Antibodies
Auxins
Cellular and Structural Biology
Chlorides
COMPOSICION QUIMICA
COMPOSITION CHIMIQUE
Corn
Endosperm
ENDOSPERMA
ENDOSPERME
ESTRUCTURA CELULAR
HIDROLISIS
HYDROLYSE
Hydrolysis
Indoleacetic acids
Insulin antibodies
Ions
PROLAMINAS
PROLAMINE
PROTEINAS
PROTEINE
Storage proteins
STRUCTURE CELLULAIRE
VIA BIOQUIMICA DEL METABOLISMO
VOIE BIOCHIMIQUE DU METABOLISME
ZEA MAYS
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Summary:Commercial zein was base-hydrolyzed and purified extracts were subjected to gas chromatography-selected ion monitoring-mass spectrometry analysis. Indoleacetic acid (IAA) was shown to be released from this storage protein of corn (Zea mays). Isotope dilution using [13C6]IAA as an internal standard revealed a conservative ratio of 1 mole IAA to 175 moles zein. Immunoelectron micrographs of isolated protein bodies also showed IAA or an IAA-like molecule associated with zein and deposited within these organelles
Bibliography:F50
F60
9146006
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:0032-0889
1532-2548
DOI:10.1104/pp.96.4.1070