Crystal structure of firefly luciferase throws light on a superfamily of adenylate-forming enzymes

• Published in: Structure (London) Vol. 4; no. 3; pp. 287 - 298
• Main Authors: Conti, Elena, Franks, Nick P, Brick, Peter
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 15.03.1996
• Subjects: acyl-coenzyme A ligase; Adenosine - metabolism; adenylate; Amino Acid Sequence; Animals; Binding Sites; Coenzyme A Ligases - metabolism; Coleoptera - chemistry; Coleoptera - enzymology; Crystallization; Crystallography, X-Ray; firefly luciferase; Luciferases - chemistry; Molecular Sequence Data; Molecular Structure; Peptide Synthases - metabolism; peptide synthetase; Protein Structure, Secondary; Stereoisomerism; X-ray crystallography; X-ray crystallography; peptide synthetase; adenylate; firefly luciferase; acyl-coenzyme A ligase
• ISSN: 0969-2126; 1878-4186
• DOI: 10.1016/S0969-2126(96)00033-0

Background: Firefly luciferase is a 62 kDa protein that catalyzes the production of light. In the presence of MgATP and molecular oxygen, the enzyme oxidizes its substrate, firefly luciferin, emitting yellow-green light. The reaction proceeds through activation of the substrate to form an adenylate intermediate. Firefly luciferase shows extensive sequence homology with a number of enzymes that utilize ATP in adenylation reactions. Results We have determined the crystal structure of firefly luciferase at 2.0 å resolution. The protein is folded into two compact domains. The large N-terminal domain consists of a β-barrel and two β-sheets. The sheets are flanked by α-helices to form an α β α β α five-layered structure. The C-terminal portion of the molecule forms a distinct domain, which is separated from the N-terminal domain by a wide cleft. Conclusion Firefly luciferase is the first member of a superfamily of homologous enzymes, which includes acyl-coenzyme A ligases and peptide synthetases, to have its structure characterized. The residues conserved within the superfamily are located on the surfaces of the two domains on either side of the cleft, but are too far apart to interact simultaneously with the substrates. This suggests that the two domains will close in the course of the reaction. Firefly luciferase has a novel structural framework for catalyzing adenylate-forming reactions.