Phenotypic and Phylogenetic Analysis of Lactic Acid Bacteria Isolated from Forage Crops and Grasses in the Tibetan Plateau

• Published in: The journal of microbiology Vol. 50; no. 1; pp. 63 - 71
• Main Authors: Pang, Huili, Zhengzhou University, Henan, P. R. China, Tan, Zhongfang, Zhengzhou University, Henan, P. R. China, Qin, Guangyong, Zhengzhou University, Henan, P. R. China, Wang, Yanping, Zhengzhou University, Henan, P. R. China, Li, Zongwei, Zhengzhou University, Henan, P. R. China, Jin, Qingsheng, Zhejiang Academy of Agricultural Sciences, Zhejiang, P. R. China, Cai, Yimin, Japan International Research Center for Agricultural Sciences, Tsukuba, Ibaraki, Japan
• Format: Journal Article
• Language: English
• Published: Heidelberg The Microbiological Society of Korea 01.02.2012; Springer Nature B.V; 한국미생물학회
• Subjects: 16S rDNA; Acids; Alfalfa; Bacteria; BACTERIAS ACIDOLACTICAS; BACTERIE LACTIQUE; Biodiversity; Biomedical and Life Sciences; Carbohydrates; Cluster Analysis; Cold regions; Community structure; Corn; Crops; Crops, Agricultural - microbiology; Dairy farms; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; epiphytic microflora; Fermentation; FORAGE; Forage crops; FORRAJES; FOURRAGE; Gene amplification; Grasses; Laboratories; LACTIC ACID BACTERIA; Lactobacillales - classification; Lactobacillales - isolation & purification; Lactobacillales - physiology; Lactobacillus brevis; Lactococcus lactis; Leuconostoc pseudomesenteroides; Life Sciences; Microbiology; Microorganisms; Molecular Sequence Data; Morphology; Natural populations; Phylogenetics; Phylogeny; Physiology; Poaceae - microbiology; Polymerase chain reaction; Rec A Recombinases - genetics; recA gene; RecA protein; Ribosomal DNA; RNA, Ribosomal, 16S - genetics; rRNA 16S; Sequence Analysis, DNA; Silage; Tibet; Weissella paramesenteroides; 생물학; Tibet; Tibetan Plateau; United States > US; China; Japan; forage; A gene; 16S rDNA; epiphytic microflora; lactic acid bacteria
• ISSN: 1225-8873; 1976-3794
• DOI: 10.1007/s12275-012-1284-5

A total of 140 lactic acid bacteria (LAB) strains were isolated from corn, alfalfa, clover, sainfoin, and Indian goosegrass in the Tibetan Plateau. According to phenotypic and chemotaxonomic characteristics, 16S rDNA sequence, and recA gene PCR amplification, these LAB isolates were identified as belonging to five genera and nine species. Corn contained more LAB species than other forage crops. Leuconostoc pseudomesenteroides, Lactococcus lactis subsp. lactis, Lactobacillus brevis, and Weissella paramesenteroides were dominant members of the LAB population on alfalfa, clover, sainfoin, and Indian goosegrass, respectively. The comprehensive 16S rDNA and recA-based approach effectively described the LAB community structure of the relatively abundant LAB species distributed on different forage crops. This is the first report describing the diversity and natural populations of LAB associated with Tibetan forage crops, and most isolates grow well at or below 10℃. The results will be valuable for the future design of appropriate inoculants for silage fermentation in this very cold area.