Effects of levosimendan and dobutamine on experimental acute lung injury in rats

• Published in: Acta histochemica Vol. 111; no. 5; pp. 404 - 414
• Main Authors: Erbüyün, Koray, Vatansever, Seda, Tok, Demet, Ok, Gülay, Türköz, Ergin, Aydede, Hasan, Erhan, Yamaç, Tekin, İdil
• Format: Journal Article
• Language: English
• Published: Germany Elsevier GmbH 01.09.2009
• Subjects: Acute Lung Injury - etiology; Acute Lung Injury - metabolism; Animals; Cardiotonic Agents - pharmacology; Dobutamine; Dobutamine - pharmacology; Hydrazones - pharmacology; Hypertension - physiopathology; Immunohistochemistry; In Situ Nick-End Labeling; Interleukin-1beta - metabolism; Levosimendan; Lung - drug effects; Lung - metabolism; Lung - pathology; Lung injury; Male; Nitric Oxide Synthase Type II - metabolism; Nitric Oxide Synthase Type III - metabolism; Pyridazines - pharmacology; Rats; Rats, Wistar; Sepsis; Tumor Necrosis Factor-alpha - metabolism; Dobutamine; Rats; Sepsis; Levosimendan; Lung injury
• ISSN: 0065-1281; 1618-0372
• DOI: 10.1016/j.acthis.2008.09.010

The effects of levosimendan on acute lung injury induced by peritonitis and abdominal hypertension in the early stages of sepsis in rats were investigated. Twenty-four adult male Wistar rats were randomized into: (1) sham, (2) subjected to abdominal hypertension and peritonitis induced lung injury using cecal ligation and puncture, then treated by dobutamine, (3) subjected to abdominal hypertension and peritonitis induced lung injury using cecal ligation and puncture, then treated by levosimendan, and (4) controls subjected to abdominal hypertension and peritonitis induced lung injury using cecal ligation and puncture with no treatment. In the control and levosimendan groups, cecal ligation and puncture resulted in moderate IL-1 β immunolabelling in lung tissue; marked IL-1 β immunolabelling was demonstrated in the dobutamine group. TNF- α immunolabelling was negative in both the sham and levosimendan groups, but moderate and weak immunoreactivities were observed in the dobutamine and control groups, respectively. There were almost no TUNEL positive cells in the sham, but they were prominent in the control. TUNEL positive cells were significantly less in the levosimendan treated lungs when compared to control and dobutamine groups. Immunoreactivity of eNOS was stronger in the dobutamine group when compared with the levosimendan group. In addition, iNOS immunoreactivity was strongly detected in the control group; this immunoreactivity was less in the levosimendan group than the dobutamine group. In this experimental sepsis model, treatment with levosimendan had a marked effect on attenuating or decreasing apoptosis and inflammation in the lung.