Cu-BTC Derived Mesoporous CuS Nanomaterial as Nanozyme for Colorimetric Detection of Glutathione

• Published in: Molecules (Basel, Switzerland) Vol. 29; no. 9; p. 2117
• Main Authors: Zhang, Xiwen, Wang, Jie, Chang, Nan, Yang, Yu, Li, Yuqi, Wei, Qi, Ni, Chang, Song, Wanying, Ma, Mingyue, Feng, Xun, Fan, Ronghua
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01.05.2024
• Subjects: Benzidines - chemistry; Carbon; Catalysis; Chemical elements; colorimetric detection; Colorimetry - methods; Copper - chemistry; Cu-BTC; Disease; Enzymes; glutathione; Glutathione - analysis; Glutathione - chemistry; Humans; Hydrogen Peroxide - analysis; Hydrogen Peroxide - chemistry; Limit of Detection; Liver diseases; mesoporous CuS nanomaterial; Morphology; Nanomaterials; Nanoparticles; Nanostructures - chemistry; nanozyme; Oxidation-Reduction; Phthalic Acids - chemistry; Pore size; Porosity; Thiols; China; colorimetric detection; mesoporous CuS nanomaterial; nanozyme; Cu-BTC; glutathione
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules29092117

In this paper, Cu-BTC derived mesoporous CuS nanomaterial (m-CuS) was synthesized via a two-step process involving carbonization and sulfidation of Cu-BTC for colorimetric glutathione detection. The Cu-BTC was constructed by 1,3,5-benzenetri-carboxylic acid (H BTC) and Cu ions. The obtained m-CuS showed a large specific surface area (55.751 m /g), pore volume (0.153 cm /g), and pore diameter (15.380 nm). In addition, the synthesized m-CuS exhibited high peroxidase-like activity and could catalyze oxidation of the colorless substrate 3,3',5,5'-tetramethylbenzidine to a blue product. Peroxidase-like activity mechanism studies using terephthalic acid as a fluorescent probe proved that m-CuS assists H O decomposition to reactive oxygen species, which are responsible for TMB oxidation. However, the catalytic activity of m-CuS for the oxidation of TMB by H O could be potently inhibited in the presence of glutathione. Based on this phenomenon, the colorimetric detection of glutathione was demonstrated with good selectivity and high sensitivity. The linear range was 1-20 μM and 20-300 μM with a detection limit of 0.1 μM. The m-CuS showing good stability and robust peroxidase catalytic activity was applied for the detection of glutathione in human urine samples.