Sialyllactose occurs as free lactones in ovine colostrum

• Published in: Biochimica et biophysica acta Vol. 1381; no. 3; pp. 286 - 292
• Main Authors: Nakamura, Tadashi, Urashima, Tadasu, Nakagawa, Miyuki, Saito, Tadao
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 24.08.1998
• Subjects: analogs & derivatives; analysis; Animals; Carbohydrate Conformation; Carbohydrate Sequence; chemistry; Chromatography, High Pressure Liquid; Colostrum; Colostrum - chemistry; Female; isolation & purification; Lactone; Lactones; Lactones - analysis; Lactones - chemistry; Lactose; Lactose - analogs & derivatives; Lactose - analysis; Lactose - isolation & purification; Magnetic Resonance Spectroscopy; Molecular Sequence Data; Molecular Structure; Oligosaccharides; Oligosaccharides - analysis; Oligosaccharides - isolation & purification; Ovine; Sheep; Sialic Acids; Sialic Acids - analysis; Sialic Acids - isolation & purification; Sialyllactose; Glc, glucose; Sialyllactose; Neu5Ac, N-acetylneuraminic acid; Neu5Gc, N-glycolylneuraminic acid; Lactone; Ovine; Gal, galactose; Colostrum
• ISSN: 0304-4165; 0006-3002; 1872-8006
• DOI: 10.1016/S0304-4165(98)00040-3

Three sialyl oligosaccharide fractions were separated from ovine colostrum by gel filtration, anion exchange chromatography and normal-phase HPLC. They were characterized by 1H-NMR spectrometry as follows: Neu5Acα2→3Galβ1→4Glc, Neu5Gcα2→6Galβ1→4Glc and three forms of Neu5Gcα2→3Galβ1→4Glc, namely Neu5Gcα2→3Galβ1→4Glc itself, its lactone derivative between the carboxyl group of Neu5Gc and Gal OH-2 and another lactone derivative between the carboxyl group and Gal OH-4. In this study, Neu5Gc-lactose lactones, in their free form, have been isolated for the first time from any natural sources including milk or colostrum.