Chemotactic Activity of CXC Chemokines Interleukin-8, Growth-Related Oncogene—α, and Epithelial Cell—Derived Neutrophil-Activating Protein—78 in Urine of Patients with Urosepsis

• Published in: The Journal of infectious diseases Vol. 182; no. 6; pp. 1731 - 1737
• Main Authors: Olszyna, Dariusz P., Opal, Steven M., Prins, Jan M., Horn, David L., Speelman, Peter, van Deventer, Sander J. H., van der Poll, Tom
• Format: Journal Article
• Language: English
• Published: Chicago, IL The University of Chicago Press 01.12.2000; University of Chicago Press
• Subjects: Acute Disease; Adult; Antibacterial agents; Antibiotics; Antibiotics. Antiinfectious agents. Antiparasitic agents; Antibodies; Antibodies - pharmacology; Bacteriology; Biological and medical sciences; Blood plasma; Chemokine CXCL5; Chemokines; Chemokines, CXC - blood; Chemokines, CXC - immunology; Chemokines, CXC - urine; CXC chemokines; ENA-78 protein; Endotoxins; Endotoxins - administration & dosage; Fundamental and applied biological sciences. Psychology; GRO-^a protein; Growth Substances - blood; Growth Substances - immunology; Growth Substances - urine; Hospital admissions; Humans; Interleukin-8 - analogs & derivatives; Interleukin-8 - blood; Interleukin-8 - immunology; Interleukin-8 - urine; Major Articles; Medical sciences; Microbiology; Neutrophil Activation; Neutrophils; Neutrophils - drug effects; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains; Pharmacology. Drug treatments; Pyelonephritis - urine; Urinary tract; Urine; Human; Urine; Growth; Cytokine; Epithelial cell; Neutrophil; Onc gene; Interleukin 8; Protein
• ISSN: 0022-1899; 1537-6613
• DOI: 10.1086/317603

CXC chemokines are chemotactic cytokines that specifically act on neutrophils. To obtain insight into the extent of local production of CXC chemokines during acute pyelonephritis, interleukin (IL)—8, growth-related oncogene (GRO)—α, and epithelial cell—derived neutrophilactivating protein (ENA)—78 were measured in urine and plasma samples from patients with culture-proven urosepsis (n = 33), healthy human control subjects with sterile urine (n = 31), and human volunteers intravenously injected with endotoxin (n = 11). Patients had profoundly elevated urine concentrations of chemokines with no (GRO-α and ENA-78) or little (IL-8) elevation in plasma. Endotoxin-challenged subjects demonstrated transient increases in plasma chemokine concentrations, with no (GRO-α) or little (IL-8 and ENA-78) elevation in urine. Urine from patients exerted chemotactic activity toward neutrophils, which was partially inhibited by neutralizing antibodies against IL-8, GRO-α, or ENA-78. During urosepsis, CXC chemokines are predominantly produced within the urinary tract, where they are involved in the recruitment of neutrophils to the urinary compartment.