Microfluidic Based Optical Microscopes on Chip

• Published in: Cytometry. Part A Vol. 93; no. 10; pp. 987 - 996
• Main Authors: Paiè, Petra, Martínez Vázquez, Rebeca, Osellame, Roberto, Bragheri, Francesca, Bassi, Andrea
• Format: Journal Article
• Language: English
• Published: Hoboken, USA John Wiley & Sons, Inc 01.10.2018; Wiley Subscription Services, Inc
• Subjects: Automation; Biomonitoring; brightfield; Cell culture; Fluorescence; Fluorescence microscopy; Imaging; Imaging techniques; lab on chip; Lab-on-a-chip; Microfluidics; Microscopes; Optical components; Optical microscopes; optical microscopy; Optics; optofluidics; Phase contrast; Review; lab on chip; brightfield; fluorescence; optofluidics; optical microscopy
• ISSN: 1552-4922; 1552-4930; 1552-4930
• DOI: 10.1002/cyto.a.23589

Last decade's advancements in optofluidics allowed obtaining an ever increasing integration of different functionalities in lab on chip devices to culture, analyze, and manipulate single cells and entire biological specimens. Despite the importance of optical imaging for biological sample monitoring in microfluidics, imaging is traditionally achieved by placing microfluidics channels in standard bench‐top optical microscopes. Recently, the development of either integrated optical elements or lensless imaging methods allowed optical imaging techniques to be implemented in lab on chip systems, thus increasing their automation, compactness, and portability. In this review, we discuss known solutions to implement microscopes on chip that exploit different optical methods such as bright‐field, phase contrast, holographic, and fluorescence microscopy.