Biotransformation of catechin and extraction of active polysaccharide from green tea leaves via simultaneous treatment with tannase and pectinase
BACKGROUND Green tea is a dietary source of bioactive compounds for human health. Enzymatic treatments induce the bioconversion of bioactive components, which can improve biological activities. In this study, we investigated the effect of simultaneous treatment with tannase and Rapidase on biotransf...
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Published in | Journal of the science of food and agriculture Vol. 95; no. 11; pp. 2337 - 2344 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Chichester, UK
John Wiley & Sons, Ltd
30.08.2015
John Wiley and Sons, Limited |
Subjects | |
Online Access | Get full text |
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Summary: | BACKGROUND
Green tea is a dietary source of bioactive compounds for human health. Enzymatic treatments induce the bioconversion of bioactive components, which can improve biological activities. In this study, we investigated the effect of simultaneous treatment with tannase and Rapidase on biotransformation of catechins and extraction of polysaccharide from green tea extract (GTE).
RESULTS
Tannase and pectinase treatments induced the biotransformation of catechins and altered tea polysaccharide () content. The addition of GTE to the enzyme reaction resulted in a significant increase in degallated catechins, including gallic acid, a product of the tannase reaction (314.5–4076.0 µg mL−1) and a reduction in epigallocatechin gallate (EGCG). Biotransformation of catechins improved the radical scavenging activity of GTE. Pectinase treatment led to change of TPS composition in GTE by hydrolyzing polysaccharides. In addition, pectinase‐driven hydrolysis in polysaccharides significantly increased TPS‐induced Interleukin 6 (IL‐6) production in macrophages. In particular, treatment of Rapidase (TPS‐Ra) led to the highest IL‐6 production among TPS samples, similar to treatment of highly purified pectinase (TPS‐GTE), a positive control.
CONCLUSION
Simultaneous processing with tannase and Rapidase can be an efficient method for the extraction of bioactive polysaccharides and biotransformation of catechins with enhanced radical scavenging activity from green tea. © 2014 Society of Chemical Industry |
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Bibliography: | ark:/67375/WNG-9LRBCTFD-0 istex:BA3EFB591F333A385F48ACF69DE4F1673AB7DFC2 ArticleID:JSFA6955 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0022-5142 1097-0010 |
DOI: | 10.1002/jsfa.6955 |