Simultaneous determination of sulfasalazine and its metabolites sulfapyridine and N-acetylsulfapyridine in human serum by ion-pair high-performance liquid chromatography using a polymer-based column

An HPLC method is described for the simultaneous and rapid determination of sulfasalazine (salicylazosulfapyridine) and two of its metabolites, sulfapyridine and N-acetylsulfapyridine, in human serum. The range of quantitation is 0.1 to 12 micrograms/mL for sulfasalazine and sulfapyridine and 0.25 t...

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Bibliographic Details
Published inJournal of pharmaceutical sciences Vol. 79; no. 12; p. 1095
Main Authors Buggé, C J, Gautam, S R, Parke, L E, Mason, J T, Garcia, D B
Format Journal Article
LanguageEnglish
Published United States 01.12.1990
Subjects
Acetonitriles - analysis
Administration, Oral
Chromatography, High Pressure Liquid - methods
Humans
Ions
Quality Control
Sulfapyridine - analogs & derivatives
Sulfapyridine - blood
Sulfasalazine - blood
Time Factors
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Summary:An HPLC method is described for the simultaneous and rapid determination of sulfasalazine (salicylazosulfapyridine) and two of its metabolites, sulfapyridine and N-acetylsulfapyridine, in human serum. The range of quantitation is 0.1 to 12 micrograms/mL for sulfasalazine and sulfapyridine and 0.25 to 12 micrograms/mL for N-acetylsulfapyridine. Serum is mixed with acetonitrile containing the internal standard sulfamethazine and the ion-pairing agent tetraethylammonium chloride. The acetonitrile extract is concentrated and analyzed by HPLC, using a new polymer-based column, and detected by UV spectroscopy at 270 nm. This paper is the first both to describe the simultaneous analysis of all three of the compounds from serum and to present sulfasalazine concentration-time data following oral administration to humans.
ISSN:0022-3549
DOI:10.1002/jps.2600791211