role of aminopeptidase PepS in the growth of Streptococcus thermophilus is not restricted to nitrogen nutrition

• Published in: Journal of applied microbiology Vol. 108; no. 1; pp. 148 - 157
• Main Authors: Thomas, S, Besset, C, Courtin, P, Rul, F
• Format: Journal Article
• Language: English
• Published: Oxford, UK Oxford, UK : Blackwell Publishing Ltd 2010; Blackwell Publishing Ltd; Blackwell; Wiley
• Subjects: aminopeptidase; Aminopeptidases - metabolism; Anti-Infective Agents - pharmacology; bacterial growth; Bacterial Proteins - metabolism; Biological and medical sciences; Culture Media; Drug Resistance, Bacterial - physiology; Fundamental and applied biological sciences. Psychology; Gene Deletion; Hot Temperature; lactic acid bacteria; Life Sciences; lysozyme resistance; Metalloendopeptidases - metabolism; Microbiology; Muramidase - pharmacology; Nitrogen - metabolism; nitrogen nutrition; Streptococcus thermophilus; Streptococcus thermophilus - enzymology; Streptococcus thermophilus - genetics; Streptococcus thermophilus - growth & development; Lactic acid bacteria; Streptococcus thermophilus; Nutrition; Enzyme; Growth; Applied microbiology; Aminopeptidases; Nitrogen; lysozyme resistance; nitrogen nutrition; bacterial growth; Glycosylases; Resistance; Peptidases; Streptococcaceae; Streptococcus salivarius subsp. thermophilus; Glycosidases; aminopeptidase; Bacteria; Hydrolases; Micrococcales; Lysozyme
• ISSN: 1364-5072; 1365-2672
• DOI: 10.1111/j.1365-2672.2009.04400.x

To investigate the effect of an absence of aminopeptidase PepS on the growth of Streptococcus thermophilus on different media and at different temperatures. Using gene interruption, a negative mutant of the Strep. thermophilus CNRZ385 strain was constructed for the aminopeptidase PepS (strain ΔpepS). Checks were first of all made using biochemical assays that the ΔpepS strain lacks the peptide hydrolase activity of aminopeptidase PepS. It was demonstrated that the absence of the aminopeptidase PepS exerted a negative effect on growth whatever the culture medium (M17, chemically defined medium, milk). The role of aminopeptidase PepS in growth was enhanced at a high temperature (45°C vs 37°C). The ΔpepS strain was more resistant to lysozyme than the wild-type strain. We were able to demonstrate that aminopeptidase PepS probably plays a pleiotropic role through its involvement in growth via nitrogen nutrition, as well as via other cellular functions/metabolisms (such as peptidoglycane metabolism). This study constitutes the first report on the role of a member of the M29 MEROPS family of metallopeptidases ( http://merops.sanger.ac.uk/).