Permethrin Alters Adipogenesis in 3T3-L1 Adipocytes and Causes Insulin Resistance in C2C12 Myotubes

• Published in: Journal of biochemical and molecular toxicology Vol. 28; no. 9; pp. 418 - 424
• Main Authors: Kim, Jonggun, Park, Yooheon, Yoon, Kyong Sup, Clark, J. Marshall, Park, Yeonhwa
• Format: Journal Article
• Language: English
• Published: United States Blackwell Publishing Ltd 01.09.2014; Wiley Subscription Services, Inc
• Subjects: 3T3-L1 Cells; Adipocytes; Adipocytes - metabolism; Adipocytes - pathology; Adipogenesis; Adipogenesis - drug effects; Animals; Biomarkers - metabolism; Gene Expression Regulation - drug effects; Glucose; Glucose - metabolism; Homeostasis; Humans; Insecticides - adverse effects; Insecticides - pharmacology; Insulin; Insulin Resistance; Lipids; Mice; Muscle Fibers, Skeletal - metabolism; Muscle Fibers, Skeletal - pathology; Permethrin; Permethrin - adverse effects; Permethrin - pharmacology; Pyrethroids; Pyrethroids; Permethrin; Glucose Metabolism; Adipogenesis
• ISSN: 1095-6670; 1099-0461
• DOI: 10.1002/jbt.21580

ABSTRACT Pyrethroids are a class of insecticides structurally derived from the naturally occurring insecticides called pyrethrins. Along with emerging evidence that exposure to insecticides is linked to altered weight gain and glucose homeostasis, exposure to pyrethroids has been linked to altered blood glucose levels in humans. Thus, the purpose of this study was to determine the role of permethrin on lipid and glucose metabolisms. Permethrin was treated to 3T3‐L1 adipocytes and C2C12 myoblasts to determine its role in lipid and glucose metabolisms, respectively. Permethrin treatment resulted in increased expression of key markers of adipogenesis and lipogenesis in adipocytes. Permethrin significantly reduced insulin‐stimulated glucose uptake in myotubes. This is the first report on the role of permethrin in altered lipid metabolism in adipocytes and impaired glucose homeostasis in myotubes. These results may help elucidate fundamental underlying mechanisms between insecticide exposure, particularly permethrin, and potential risk of developing obesity and its comorbidities.