Effect of Organic Selenium in the Diet on Sperm Quality of Boars

• Published in: Reproduction in domestic animals Vol. 45; no. 6; pp. e297 - e305
• Main Authors: López, A, Rijsselaere, T, Van Soom, A, Leroy, JLMR, De Clercq, JBP, Bols, PEJ, Maes, D
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.12.2010
• Subjects: Animal Feed - analysis; Animal Nutritional Physiological Phenomena; Animals; Diet - veterinary; Dietary supplements; Hogs; Male; Organic chemicals; Selenium; Selenium - chemistry; Selenium - pharmacology; Semen - physiology; Sperm Motility - drug effects; Spermatozoa - drug effects; Spermatozoa - physiology; Swine - blood; Swine - physiology; Thiobarbituric Acid Reactive Substances; Vitamin E
• ISSN: 0936-6768; 1439-0531
• DOI: 10.1111/j.1439-0531.2009.01560.x

The effect of a diet supplemented with organic selenium (Se) on sperm production and quality of boars was investigated. Sixty mature boars from a commercial artificial insemination centre were randomly allocated at Day (D) 0 into Group A and B. Group A received the regular ration supplemented with inorganic Se (0.4 mg/kg feed as Na₂SeO₃) whereas Group B was switched to the same diet but with organic Se (0.4 mg/kg fed as Se‐yeast). The sperm was investigated during 4 months (D0, D30, D60, D75, D90, D105 and D120). Sperm concentration and motility were objectively measured using a photometer and Computer Assisted Semen Analysis (CASA) respectively. Morphology of the sperm was assessed using eosin-nigrosin staining and the resistance to induction of oxidative stress (production of malonaldehyde, MDA) through thiobarbituric acid reagent species analysis. Additionally, the Se concentration in sperm and blood plasma were measured. Repeated measures analysis of variance (anova) from D60 to 120 (spermatogenesis of approximately 2 months) or anova at D120 (Se concentrations) were used for statistical analysis. The total number of ejaculated sperm was not significantly different between both groups, but boars of Group B had a significantly higher sperm concentration (434.6 vs 514.1 × 10⁶ sperm/ml; p < 0.05). Small differences (p < 0.05) were observed between both groups for some CASA parameters, namely straight line velocity (μm/s) (Group A: 48.3, Group B: 45.1), straightness (%) (Group A: 65.6, Group B: 62.2) and linearity (%) (Group A: 32.2, Group B: 29.3). The sperm of Group B showed more oxidative stress (4.1 vs 4.9 μmol MDA/l; p < 0.05) compared with those of Group A. No significant differences (p > 0.05) were observed for the other parameters. Under the present study conditions, changing from inorganic Se to organic Se in the diet of boars increased sperm concentration but reduced some motility parameters and resistance to oxidative stress.