Epithelial and Endothelial Pannexin1 Channels Mediate AKI

• Published in: Journal of the American Society of Nephrology Vol. 29; no. 7; pp. 1887 - 1899
• Main Authors: Jankowski, Jakub, Perry, Heather M, Medina, Christopher B, Huang, Liping, Yao, Junlan, Bajwa, Amandeep, Lorenz, Ulrike M, Rosin, Diane L, Ravichandran, Kodi S, Isakson, Brant E, Okusa, Mark D
• Format: Journal Article
• Language: English
• Published: United States American Society of Nephrology 01.07.2018
• Subjects: Acute Kidney Injury - etiology; Acute Kidney Injury - metabolism; Acute Kidney Injury - prevention & control; Adenosine Triphosphate - metabolism; Animals; Anti-Ulcer Agents - pharmacology; Basic Research; Bone Marrow Cells - metabolism; Carbenoxolone - pharmacology; Connexins - antagonists & inhibitors; Connexins - genetics; Cytokines - metabolism; Disease Models, Animal; Endothelial Cells - metabolism; Endothelium, Vascular; Epithelial Cells - metabolism; Intercellular Adhesion Molecule-1 - metabolism; Male; Mice; Mice, Knockout; Nerve Tissue Proteins - antagonists & inhibitors; Nerve Tissue Proteins - genetics; Reperfusion Injury - genetics; Reperfusion Injury - metabolism; Reperfusion Injury - pathology; Reperfusion Injury - prevention & control; RNA, Messenger - metabolism; purinergic signaling; acute renal failure; ischemia-reperfusion; ATP
• ISSN: 1046-6673; 1533-3450
• DOI: 10.1681/asn.2017121306

Pannexin1 (Panx1), an ATP release channel, is present in most mammalian tissues, but the role of Panx1 in health and disease is not fully understood. Panx1 may serve to modulate AKI; ATP is a precursor to adenosine and may function to block inflammation, or ATP may act as a danger-associated molecular pattern and initiate inflammation. We used pharmacologic and genetic approaches to evaluate the effect of Panx1 on kidney ischemia-reperfusion injury (IRI), a mouse model of AKI. Pharmacologic inhibition of gap junctions, including Panx1, by administration of carbenoxolone protected mice from IRI. Furthermore, global deletion of preserved kidney function and morphology and diminished the expression of proinflammatory molecules after IRI. Analysis of bone marrow chimeric mice revealed that Panx1 expressed on parenchymal cells is necessary for ischemic injury, and both proximal tubule and vascular endothelial tissue-specific knockout mice were protected from IRI. , -deficient proximal tubule cells released less and retained more ATP under hypoxic stress. Panx1 is involved in regulating ATP release from hypoxic cells, and reducing this ATP release may protect kidneys from AKI.