Analysis of a continuous culture of Fibrobacter succinogenes S85 on a standardized glucose medium

• Published in: Bioprocess and biosystems engineering Vol. 33; no. 4; pp. 417 - 425
• Main Authors: Guiavarch, E, Pons, A, Christophe, G, Creuly, C, Dussap, C.-G
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Berlin/Heidelberg : Springer-Verlag 01.05.2010; Springer-Verlag; Springer; Springer Nature B.V
• Subjects: Anaerobiosis; Bioengineering; Biological and medical sciences; Biomass; Bioreactors; Bioreactors - microbiology; Biotechnology; Carbon Dioxide - metabolism; Carbon sources; Chemistry; Chemistry and Materials Science; Culture Media, Serum-Free; Environmental Engineering/Biotechnology; Equipment Design; Fibrobacter - growth & development; Fibrobacter - metabolism; Fibrobacter succinogenes; Food Science; Fundamental and applied biological sciences. Psychology; Glucose; Glucose - metabolism; Industrial and Production Engineering; Industrial Chemistry/Chemical Engineering; Mathematical models; Microbiology; Models, Biological; Original Paper; Oxidation-Reduction; Plants; Redox potential; Waste Products; Glucose; Redox potential; Stoichiometric analysis; CO; Continuous; partial pressure; Fibrobacter succinogenes; Carbon dioxide; Fibrobacter; Pressure; Bacteroidaceae; Continuous process; Bacteria; Culture
• ISSN: 1615-7591; 1615-7605
• DOI: 10.1007/s00449-009-0342-9

Continuous cultures of Fibrobacter succinogenes S85 were performed on a standardized fully synthetic culture medium with glucose as carbon source at a dilution rate (D = 0.02 h⁻¹) in a 5-L bioreactor. The culture was stabilized during 20 days and demonstrated the ability of Fibrobacter succinogenes to grow in this synthetic medium. CO₂ partial pressure and redox potential probes were used to check the anaerobic state of the culture. The biomass yield was calculated 0.206 g (g glucose)⁻¹ and the production yield of succinate, the major end-product, was 0.63 mol (mol glucose)⁻¹. The consistency of the experimental data was checked by proton and mass (C, N) balances. The results were satisfactory (90-110% recovery) leading to derive a stoichiometric equation representative of the growth on glucose. The stoichiometric coefficients were calculated using data reconciliation and linear algebra methods enabling to obtain a complete modeling of all conversion yields possible.