Expression of pattern recognition receptors and activation of the non-canonical inflammasome pathway in brain pericytes

• Published in: Brain, behavior, and immunity Vol. 64; pp. 220 - 231
• Main Authors: Nyúl-Tóth, Ádám, Kozma, Mihály, Nagyőszi, Péter, Nagy, Krisztina, Fazakas, Csilla, Haskó, János, Molnár, Kinga, Farkas, Attila E, Végh, Attila G, Váró, György, Galajda, Péter, Wilhelm, Imola, Krizbai, István A
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Inc 01.08.2017
• Subjects: Allergy and Immunology; Blood–brain barrier (BBB); Brain - metabolism; Cells, Cultured; Cerebral pericyte; Gene Expression Regulation; Humans; IL-1β; Inflammasome; Inflammasomes - metabolism; Interleukin-1beta - metabolism; NOD-like receptor (NLR); Non-canonical; pattern recognition receptor (PRR); Pericytes - metabolism; Psychiatry; Receptors, Pattern Recognition - metabolism; Signal Transduction; Toll-like receptor (TLR); CXCL; granulocyte-macrophage colony stimulating factor; BBB; RANTES; Escherichia coli; MHC; ICAM-1; Inflammasome; major histocompatibility complex; NVU; CARD; nucleotide-binding oligomerization domain; PRR; IFN-γ; TBS-T; regulated on activation, normal t cell expressed and secreted; GM-CSF; HBVP; bloodbrain barrier; damage-associated molecular pattern; MDP; iNOS; pattern recognition receptor (PRR); Toll-like receptor; intercellular adhesion molecule-1; CEC; NOD; IL; standard error of mean; IP-10; IL-1β; G-CSF; green fluorescent protein; central nervous system; C-X-C motif ligand; macrophage inflammatory protein-1α; IFNγ-induced protein-10; outer membrane vesicle; AIM2; enzyme linked immuno sorbent assay; Toll-like receptor (TLR); C-C motif ligand; cerebral endothelial cell; OMV; SEM; lipopolysaccharide; GAPDH; ELISA; glyceraldehyde 3-phosphate dehydrogenase; interferon-γ; MCP-1; Cerebral pericyte; monocyte chemoattractant protein-1; CNS; Blood–brain barrier (BBB); apoptosis-associated speck-like protein containing CARD; LPS; vascular cell adhesion molecule-1; inducible nitric oxide synthase; GFP; PDGFRβ; NOD-like receptor (NLR); ASC; caspase activation and recruitment domain; Non-canonical; NLR; MIP-1α; TLR; interleukin; pathogen-associated molecular pattern; platelet-derived growth factor receptor β; E. coli; neurovascular unit; NOD-like receptor; muramyl dipeptide; human brain vascular pericyte; Tris-buffered saline with 1 mL/L Tween-20; CCL; absent in melanoma 2; PAMP; VCAM-1; TNF-α; tumor necrosis factor-α; adenosine triphosphate; ATP; pattern recognition receptor; DAMP; granulocyte colony stimulating factor
• ISSN: 0889-1591; 1090-2139
• DOI: 10.1016/j.bbi.2017.04.010

Highlights • Brain pericytes express NOD-like and Toll-like pattern recognition receptors. • Expression of NLRs and TLRs is regulated by inflammatory mediators. • No canonical inflammasome activation could be detected in human cerebral pericytes. • Pericytes secrete active IL-1β in response to non-canonical inflammasome activation. • Pericytes might have an important regulatory role in neuroinflammation.