Specialty Grand Challenge for Molecular Signalling and Pathways in Molecular Neuroscience

• Published in: Frontiers in molecular neuroscience Vol. 14; p. 694776
• Main Author: Taymans, Jean-Marc
• Format: Journal Article
• Language: English
• Published: Lausanne Frontiers Research Foundation 24.05.2021; Frontiers Media; Frontiers Media S.A
• Subjects: chemical biology; Gene expression; Genomes; Kinases; Life Sciences; Ligands; molecular manipulation; Molecular Neuroscience; Nervous system; network; Neurosciences; omic analyses; Phage display; pharmacology; Phosphorylation; Post-translation; Protein purification; Proteins; Proteomes; Signal transduction; synapse; Transcriptomes; chemical biology pharmacology molecular manipulation network omic analyses synapse protein complex upstream/downstream process; chemical biology; upstream/downstream process; pharmacology; synapse; protein complex; molecular manipulation; omic analyses; network
• ISSN: 1662-5099; 1662-5099
• DOI: 10.3389/fnmol.2021.694776

[...]we have access to such fundamental knowledge as genome sequences (for humans as well as for multiple other species including many that are used as model organisms) (Lander et al., 2001; Venter et al., 2001), databases with tissue-specific gene expression patterns (GTEx, Protein Atlas) (GTEx Consortium, 2013; Uhlén et al., 2015), databases with key post-translational modifications on proteins to name just a few. Interactomics screening technologies are available to identify binding partners of focus proteins such as yeast 2 hybrid screening, phage display, mass spectrometric analysis of protein complexes isolated by affinity purification under native conditions, to name a few. [...]information provides a first molecular basis for modulation of the signalling pathway. Besides direct interactors, there are other partners of signalling proteins than can be discerned in a signalling cascade, such as upstream regulators and downstream partners. The ultimate goal is to gain a full picture of the pathway under study, how it is linked to nervous system functions and the equilibria that exist within the pathways and in relation to other pathways under normal and pathological conditions. Besides individual protein focused approaches, other approaches in elucidating signalling pathways are based on transcriptome-wide, proteome-wide or similar Omics approaches.