ITGA4 Gene Methylation Status in Chronic Lymphocytic Leukemia

• Published in: Future science OA Vol. 6; no. 7; p. FSO583
• Main Authors: Attia, Hanaa RM, Ibrahim, Mona Hamed, El-Aziz, Shereen H Abd, Hassan, Naglaa M, Osman, Randa A, Hagag, Heba A, Yassa, Marianne E, Abdelrahman, Amany H, Salama, Iman I, Sobeih, Mohamed Emam
• Format: Journal Article
• Language: English
• Published: England Future Science Ltd 01.08.2020; Taylor & Francis Group
• Subjects: beta 2 microglobulin; chronic lymphocytic leukemia; cytogenetics; ITGA4 gene expression; methylation analysis; prognostic markers; beta 2 microglobulin; chronic lymphocytic leukemia; prognostic markers; pyrosequencing; cytogenetics; methylation analysis; ITGA4 gene expression
• ISSN: 2056-5623; 2056-5623
• DOI: 10.2144/fsoa-2020-0034

We aimed to investigate gene expression pattern and to explore its methylation heterogeneity in chronic lymphocytic leukemia (CLL). Eighty one CLL patients and 75 healthy subjects were enrolled and prognostic evaluation of patients was assessed. q-realtime PCR was performed using Applied Biosystems, TaqMan gene expression assay. gene-specific CpG methylation was investigated in real time using pyrosequencing technology. was differentially expressed in CLL patients. The CpG sites-1, 2 and 3 showed significantly higher mean levels than healthy controls (p = <0.001, 0.007 and 0.009). Significant association between CpG site-1 and CLL has been detected using age-adjusted logistic regression (p < 0.001). Hypermethylation at gene CpG sites (1,2,3) is a characteristic feature in CLL.