Genomic diversity in Fructobacillus spp. isolated from fructose-rich niches

• Published in: PloS one Vol. 18; no. 2; p. e0281839
• Main Authors: Mohamed, Florencia, Ruiz Rodriguez, Luciana G, Zorzoli, Azul, Dorfmueller, Helge C, Raya, Raúl R, Mozzi, Fernanda
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 16.02.2023; Public Library of Science (PLoS)
• Subjects: Acids; Adaptation; Amino acids; Bacteria; Biology and Life Sciences; Comparative analysis; Computer and Information Sciences; CRISPR; Dehydrogenases; Diabetes; Ethanol; Fermentation; Food; Fructose; Fructose - metabolism; Genes; Genomes; Genomic analysis; Genomics; Glucose; Irritable bowel syndrome; Lactic acid; Lactic acid bacteria; Lactobacillales - genetics; Leuconostocaceae - genetics; Leuconostocaceae - metabolism; Metabolism; Metabolites; Nitrogen compounds; Phylogeny; Physical Sciences; United States > US; Indianapolis Indiana
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0281839

The Fructobacillus genus is a group of obligately fructophilic lactic acid bacteria (FLAB) that requires the use of fructose or another electron acceptor for their growth. In this work, we performed a comparative genomic analysis within the genus Fructobacillus by using 24 available genomes to evaluate genomic and metabolic differences among these organisms. In the genome of these strains, which varies between 1.15- and 1.75-Mbp, nineteen intact prophage regions, and seven complete CRISPR-Cas type II systems were found. Phylogenetic analyses located the studied genomes in two different clades. A pangenome analysis and a functional classification of their genes revealed that genomes of the first clade presented fewer genes involved in the synthesis of amino acids and other nitrogen compounds. Moreover, the presence of genes strictly related to the use of fructose and electron acceptors was variable within the genus, although these variations were not always related to the phylogeny.