Regulation of Cell Division and Growth in Roots of Lactuca sativa L. Seedlings by the Ent-Kaurene Diterpenoid Rabdosin B

• Published in: Journal of chemical ecology Vol. 36; no. 5; pp. 553 - 563
• Main Authors: Ding, Lan, Jing, Hongwei, Qin, Bo, Qi, Linlin, Li, Jing, Wang, Tao, Liu, Guoan
• Format: Journal Article
• Language: English
• Published: New York New York : Springer-Verlag 01.05.2010; Springer-Verlag; Springer; Springer Nature B.V
• Subjects: Agriculture; Animal and plant ecology; Animal, plant and microbial ecology; Applied ecology; Biochemistry; Biological and medical sciences; Biological Microscopy; Biomedical and Life Sciences; Cell cycle; Cell Division; Comet Assay; Deoxyribonucleic acid; Diterpenes - isolation & purification; Diterpenes - pharmacology; DNA; DNA Damage; Ecology; Ecotoxicology, biological effects of pollution; Entomology; Fundamental and applied biological sciences. Psychology; General aspects; Isodon - chemistry; Lactuca - drug effects; Lactuca - growth & development; Lactuca sativa; Life Sciences; Mitosis; Plant growth; Plant Roots - drug effects; Plant Roots - growth & development; Seedlings - drug effects; Seedlings - growth & development; Seeds; kaurene diterpenoid; Cell cycle arrest; L; Root growth; DNA damage; Root hair; Toxicity; Growth; Plant juvenile growth stage; Genotoxicity; Compositae; Ent-kaurene diterpenoid; Dicotyledones; Angiospermae; Cell cycle; Damage; Lactuca sativa; Growth inhibitor; Terpenoid; Lactuca sativa L; Ecotoxicology; Hydrocarbon; Root; Allelopathy; Cell division; Volatile organic compound; Vegetable crop; Labiatae; DNA; Diterpene; Spermatophyta; Interspecific relation
• ISSN: 0098-0331; 1573-1561
• DOI: 10.1007/s10886-010-9783-5

Rabdosin B, an ent-kaurene diterpenoid purified from the air-dried aerial parts of Isodon japonica (Burm.f) Hara var. galaucocalyx (maxin) Hara, showed a biphasic, dose-dependent effect on root growth and a strong inhibitory effect on root hair development in lettuce seedlings (Lactuca sativa L.). Lower concentrations of rabdosin B (20-80 μM) significantly promoted root growth, but its higher levels at 120-200 μM, by contrast, had inhibitory effects. Additionally, all tested concentrations (10-40 μM) inhibited root hair development of seedlings in a dose-dependent manner. Further investigations on the underlying mechanism revealed that the promotion effect of rabdosin B at the lower concentrations resulted from increasing the cell length in the mature region and enhancing the mitotic activity of meristematic cells in seedlings' root tips. In contrast, rabdosin B at higher concentrations inhibited root growth by affecting both cell length in the mature region and division of meristematic cells. Comet assay and cell cycle analysis demonstrated that the decrease of mitotic activity of root meristematic cells was due to DNA damage induced cell cycle retardation of the G₂ phase and S phase at different times.