Characterizations of sphingosylphosphorylcholine-induced scratching responses in ICR mice using naltrexon, capsaicin, ketotifen and Y-27632
Sphingosylphosphorylcholine (SPC) is upregulated in the stratum corneum of atopic dermatitis patients by sphingomyelin deacylase. We conducted an investigation, both to confirm that intradermal injection of SPC elicits scratching in mice, and to elucidate the detailed mechanism of the SPC-induced it...
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Published in | European journal of pharmacology Vol. 583; no. 1; pp. 92 - 96 |
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Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Amsterdam
Elsevier B.V
31.03.2008
Elsevier |
Subjects | |
Online Access | Get full text |
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Summary: | Sphingosylphosphorylcholine (SPC) is upregulated in the stratum corneum of atopic dermatitis patients by sphingomyelin deacylase. We conducted an investigation, both to confirm that intradermal injection of SPC elicits scratching in mice, and to elucidate the detailed mechanism of the SPC-induced itch–scratch response. Intradermal administration of SPC increased the incidence of scratching behavior in a dose-dependent manner. SPC-induced scratching could be suppressed, significantly, by the μ-opoid receptor antagonist, naltrexon, the vaniloid receptor agonist, capsaicin, and the histamine H
1 receptor antagonist ketotifen.
d-
erythro SPC, one of the SPC stereotypes, could elicit the scratch response, but not
l-
threo SPC. Y-27632 (1 mg/kg, an inhibitor of Rho-associated protein kinase (ROCK)), was found to suppress SPC-induced scratching. Both the stereospecificity of SPC and the involvement of the Rho/ROCK pathway suggested that SPC-induced scratching is related to the receptor. |
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ISSN: | 0014-2999 1879-0712 |
DOI: | 10.1016/j.ejphar.2008.01.005 |