The Absence of uPAR Is Associated with the Progression of Dermal Fibrosis

• Published in: Journal of investigative dermatology Vol. 128; no. 12; pp. 2792 - 2797
• Main Authors: Kanno, Yosuke, Kaneiwa, Aki, Minamida, Misato, Kanno, Miho, Tomogane, Kanji, Takeuchi, Koji, Okada, Kiyotaka, Ueshima, Shigeru, Matsuo, Osamu, Matsuno, Hiroyuki
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 01.12.2008; Nature Publishing Group; Elsevier Limited
• Subjects: Animals; Biological and medical sciences; Cell Proliferation; Collagen - metabolism; Dermatology; Extracellular Matrix - metabolism; Female; Fibrinolysis; Fibroblasts - metabolism; Fibrosis - pathology; Male; Medical sciences; Mice; Mice, Transgenic; Microscopy, Electron, Scanning; Models, Biological; Receptors, Urokinase Plasminogen Activator - genetics; Receptors, Urokinase Plasminogen Activator - physiology; Skin - pathology; Association; Prognosis; Dermatology; Fibrosis; Evolution; Skin; Derm
• ISSN: 0022-202X; 1523-1747
• DOI: 10.1038/jid.2008.157

The fibrinolytic system is considered to play an important role in the degradation of extracellular matrices (ECM). However, the detailed mechanism regarding how this system affects fibrosis remains unclear. Urokinase-type plasminogen activator receptor (uPAR) not only functions as a proteinase receptor but also plays a role in cellular adhesion, differentiation, proliferation, and migration through intracellular signaling. To investigate the effect of uPAR on dermal fibrosis, the skin of wild-type mice was compared with uPAR-deficient (uPAR−/−) mice. The results showed that the absence of uPAR increases dermal thickness. In addition, collagen synthesis as well as the number of myofibroblasts was greater in the skin of uPAR−/− mice than in the skin of uPAR+/+ mice. Moreover, we showed that the absence of uPAR attenuates the activity of matrix metalloproteinases (MMP)-2, 9 in the skin. In conclusion, this study suggests that the absence of uPAR not only regulates fibrosis-related gene expression and MMP activity but also results in ECM deposition. Therefore, the absence of uPAR induces dermal fibrosis. These findings provide new insights into the role of uPAR on dermal fibrosis.