Immunoproteomic studies on paediatric opsoclonus-myoclonus associated with neuroblastoma

• Published in: Journal of neuroimmunology Vol. 297; pp. 98 - 102
• Main Authors: Torres-Vega, Estefanía, Durán-Moreno, María, del Pino, Manuel Sánchez, Yáñez, Yania, Cañete, Adela, Castel, Victoria, López-Cuevas, Rogelio, Vílchez, Juan Jesús, Dalmau, Josep, Graus, Francesc, García Verdugo, José Manuel, Bataller, Luis
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15.08.2016
• Subjects: Allergy and Immunology; Animals; Brain Neoplasms - complications; Brain Neoplasms - immunology; Brain Neoplasms - pathology; Cell Adhesion Molecules, Neuronal - metabolism; Cell Line, Tumor; Central Nervous System - metabolism; Central Nervous System - ultrastructure; Child; Databases, Factual - statistics & numerical data; Encephalitis - complications; Encephalitis - immunology; Female; HEK293 Cells; Humans; Immunoproteomics; Male; Membrane Proteins - metabolism; Nerve Tissue Proteins - metabolism; Nerve Tissue Proteins - ultrastructure; Neuroblastoma - complications; Neuroblastoma - immunology; Neuroblastoma - pathology; Neurology; Nuclear Proteins - metabolism; Nuclear Proteins - ultrastructure; Opsoclonus-myoclonus; Opsoclonus-Myoclonus Syndrome - complications; Opsoclonus-Myoclonus Syndrome - immunology; Opsoclonus-Myoclonus Syndrome - pathology; Potassium channels; Rats; Rats, Wistar; Shaw Potassium Channels - immunology; Shaw Potassium Channels - metabolism; Shaw Potassium Channels - ultrastructure; Synaptosomes; Synaptosomes - metabolism; Synaptosomes - ultrastructure; Thymoma - complications; AMPA; Kinesin family member 21; ELAV-like; Leucine-rich glioma-inactivated protein 1; Immunoproteomics; Potassium channel tetramerization domain 7; Ethylenediaminetetraacetic; Phosphate buffer saline; NMDA; Gamma-aminobutyric acid; Opsoclonus-myoclonus syndrome; N-methyl- d aspartate; Synaptosomes; Human embryonic kidney cells; Amino-3-hydroxy-5-methyl-4isoxazolepropionic acid; PBS; LGI1; SCLC; KIF21; Shaw-potassium voltage-gated channel subfamily C member 3 or 1; Embryonic lethal abnormal vision-Drosophila; Kv3.3 or 3.1; Potassium channels; EDTA; Small-cell lung cancer; HEK; KCTD7; Opsoclonus-myoclonus; OMS; GABA
• ISSN: 0165-5728; 1872-8421
• DOI: 10.1016/j.jneuroim.2016.05.015

Abstract We aimed to identify new cell-membrane antigens implicated in opsoclonus-myoclonus with neuroblastoma. The sera of 3 out of 14 patients showed IgG electron-microscopy immunogold reactivity on SH-SY5Y neuroblastoma cells. Immunoprecipitation experiments using rat brain synaptosomes and SH-SY5Y cells led to the identification of: (1) thirty-one nuclear/cytoplasmic proteins (including antigens HuB, HuC); (2) seven neuronal membrane proteins, including the Shaw-potassium channel Kv3.3 (KCNC3), whose genetic disruption in mice causes ataxia and generalized muscle twitching. Although cell-based assays did not demonstrate direct antigenicity, our findings point to Shaw-related subfamily of the potassium voltage-gated channels complexed proteins as hypothetical antigenic targets.