Highly sensitive detection of clenbuterol in urine sample by using surface plasmon resonance immunosensor

• Published in: Talanta (Oxford) Vol. 186; pp. 521 - 526
• Main Authors: Kabiraz, Dulal C., Morita, Kinichi, Sakamoto, Kazuhira, Takahashi, Masashi, Kawaguchi, Toshikazu
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15.08.2018
• Subjects: Clenbuterol; Indirect competitive inhibition; Inhibitor determination; SPR immunosensing; Urine; Urine; Indirect competitive inhibition; Clenbuterol; Inhibitor determination; SPR immunosensing
• ISSN: 0039-9140; 1873-3573
• DOI: 10.1016/j.talanta.2018.04.011

This study proposed the filtration method for removal of inhibitors from real urine samples for immunoassay without centrifuge. Although the inhibitors could not be removed by the physical filtration, the carboxyl group terminated silica effectively removed the inhibitors. In a low pH, antibody formed aggregation due to the protonation. We propose to adjust pH of the sample solution by adding a phosphate buffer solution (pH 7.5). As a result of pretreatment, the SPR immunosensing achieved the SPR signal of 45 mdeg and a low limit of detection with 100 ppq (100 fg mL−1). •It was found that urine of cow contained the inhibitor for immunoassay.•The inhibitor can be removed by carboxyl group terminated silica.•It was noticed that aggregation of antibody was formed in a low pH due to the protonation of antibody.•The SPR immunosensing with pretreatment showed the SPR signal of 45 mdeg and a low limit of detection with 0.1 ppt.