Reversal of Malignant Transformation by Tumor DNA

Walker-256 carcinosarcoma cells grown in tissue culture medium containing inhibitory DNA prepared from the tumor are shown to have an unaltered growth in vitro, but a diminished capacity to produce malignant tumors on injection into the rat. Coincident with this change in virulence is the induction...

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Bibliographic Details
Published inProceedings of the National Academy of Sciences - PNAS Vol. 67; no. 4; pp. 1827 - 1833
Main Authors Halpern, Barbara C., Halpern, Richard M., Chaney, Suzanne Q., Smith, Roberts A.
Format Journal Article
LanguageEnglish
Published United States National Academy of Sciences of the United States of America 01.12.1970
National Acad Sciences
Subjects
Acetates
Animals
Biochemistry
Biological Sciences: Biochemistry
Carcinoma 256, Walker
Cell culture techniques
Cell growth
Cell Transformation, Neoplastic - drug effects
Culture Techniques
Depression, Chemical
DNA
DNA, Neoplasm - pharmacology
Enzymes
Flasks
Methylation
Rats
RNA, Transfer
Tissue culture techniques
Transfer RNA
Transferases - antagonists & inhibitors
Transferases - metabolism
Tumors
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Summary:Walker-256 carcinosarcoma cells grown in tissue culture medium containing inhibitory DNA prepared from the tumor are shown to have an unaltered growth in vitro, but a diminished capacity to produce malignant tumors on injection into the rat. Coincident with this change in virulence is the induction of tRNA methylase inhibitors in these malignant cells. Within days after the DNA is removed from the growth medium, the tRNA methylase inhibitors disappear and the oncogenicity reappears.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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content type line 23
Chemistry Department publication number 2738. Work supported by a grant from The Julius and Dorothy Fried Research Foundation.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.67.4.1827